Gene Expression Data
Assay Details
Assay
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Reference:
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J:60196
Nokelainen P, et al., Expression of mouse 17beta-hydroxysteroid dehydrogenase/17-ketosteroid reductase type 7 in the ovary, uterus, and placenta: localization from implantation to late pregnancy. Endocrinology. 2000 Feb;141(2):772-8
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7643979
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Gene symbol:
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Hsd17b7
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Gene name:
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hydroxysteroid (17-beta) dehydrogenase 7
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Probe:
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m17HSD/KSR7 cDNA
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1A:
postnatal adult
(more )
Note: nonpregnant
Specimen
1A:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal adult
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Sex:
|
Female
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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nonpregnant
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Specimen
1D:
postnatal adult
(more )
Note: E8.5 pregnant
Specimen
1D:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal adult
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Sex:
|
Female
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
E8.5 pregnant
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Specimen
1E:
postnatal adult
(more )
Note: E14.5 pregnant
Specimen
1E:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal adult
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Sex:
|
Female
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
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Note:
|
E14.5 pregnant
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Specimen
1G:
postnatal adult
(more )
Note: E19 pregnant
Specimen
1G:
(close )
Genetic Background:
|
NMRI
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Age:
|
postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
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Note:
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E19 pregnant
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Structure |
Level |
Pattern |
Image |
Note |
TS28: corpus luteum |
Present |
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1G
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Reduced expression, or occasionally missing (data not shown).
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Specimen
1I:
postnatal adult
(more )
Note: nonpregnant
Specimen
1I:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
nonpregnant
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: corpus luteum |
Strong |
Regionally restricted |
1I
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Only some corpus lutea (active corpus luteum) were positive.
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Specimen
1J:
postnatal adult
(more )
Note: E5.5 pregnant
Specimen
1J:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
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Note:
|
E5.5 pregnant
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: corpus luteum |
Strong |
Regionally restricted |
1J
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Only some corpus lutea (active corpus luteum) were positive.
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Specimen
1K:
postnatal adult
(more )
Note: E8.5 pregnant
Specimen
1K:
(close )
Genetic Background:
|
NMRI
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Age:
|
postnatal adult
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Sex:
|
Female
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
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Note:
|
E8.5 pregnant
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Structure |
Level |
Pattern |
Image |
Note |
TS28: corpus luteum |
Very strong |
Regionally restricted |
1K
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Only some corpus lutea (active corpus luteum) were positive.
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Specimen
1L:
postnatal adult
(more )
Note: E14.5 pregnant
Specimen
1L:
(close )
Genetic Background:
|
NMRI
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Age:
|
postnatal adult
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Sex:
|
Female
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
E14.5 pregnant
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: corpus luteum |
Very strong |
|
1L
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Expressed in all large corpus lutea. No expression in a few small ones whose luteolysis and involution had further
progressed (not shown).
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Specimen
2A:
postnatal adult
(more )
Note: E5.5 pregnant
Specimen
2A:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
|
E5.5 pregnant
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Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua |
Present |
Regionally restricted |
2A
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Expressed in the stromal cells surrounding the implantation site, on the antimesometrial side. Expressed in the inner zone of decidualization.
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Specimen
2D:
postnatal adult
(more )
Note: E5.5 pregnant
Specimen
2D:
(close )
Genetic Background:
|
NMRI
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Age:
|
postnatal adult
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Sex:
|
Female
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
E5.5 pregnant
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua |
Strong |
Regionally restricted |
2D
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Expressed in the stromal cells surrounding the implantation site, on the antimesometrial side. High expression in the inner zone and low expression in the outer zone of decidualization.
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Specimen
2F:
postnatal adult
(more )
Note: E5.5 pregnant
Specimen
2F:
(close )
Genetic Background:
|
NMRI
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Age:
|
postnatal adult
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Sex:
|
Female
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
E5.5 pregnant
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua |
Weak |
Regionally restricted |
2F
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Low expression in the outer zone of the decidual reaction. No expression in the stromal edema.
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Specimen
2H:
postnatal adult
(more )
Note: E8 pregnant
Specimen
2H:
(close )
Genetic Background:
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NMRI
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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E8 pregnant
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Specimen
2H embryo:
embryonic day 8.0
(more )
Specimen
2H embryo:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2J:
postnatal adult
(more )
Note: E9.5 pregnant
Specimen
2J:
(close )
Genetic Background:
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NMRI
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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E9.5 pregnant
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Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua capsularis |
Strong |
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2J
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Expression was particularly high. No expression in the mesometrial decidual layer at E9 (not shown).
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Specimen
2J embryo:
embryonic day 9.5
(more )
Specimen
2J embryo:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3B:
embryonic day 9.0
(more )
Specimen
3B:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3C:
embryonic day 12.5
(more )
Specimen
3C:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3E:
embryonic day 14.5
(more )
Specimen
3E:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: junctional zone |
Strong |
Regionally restricted |
3E
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Expressed in spongiotrophoblasts at the junctional zone and in the spongiotrophoblast pegs within the labyrinth region.
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TS22: labyrinthine zone |
Strong |
Regionally restricted |
3E
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Expressed in spongiotrophoblasts at the junctional zone and in the spongiotrophoblast pegs within the labyrinth region.
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TS22: spongiotrophoblast |
Strong |
Regionally restricted |
3E
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Expressed in spongiotrophoblasts at the junctional zone and in the spongiotrophoblast pegs within the labyrinth region.
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Specimen
3I:
embryonic day 17.5
(more )
Specimen
3I:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS25: spongiotrophoblast |
Strong |
Regionally restricted |
3I
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Expressed in abundant spongiotrophoblasts in the peripheral region, and the region of spongiotrophoblasts that narrowed to a few cell layers between the decidual and labyrinth zones.
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Specimen
3K:
embryonic day 19.0
(more )
Specimen
3K:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 19.0
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: labyrinthine zone |
Present |
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3K
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Decreased (arrowheads) remarkably from that on E17.5.
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Specimen
not shown:
postnatal adult
(more )
Note: E4.5 pregnant
Specimen
not shown:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal adult
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Sex:
|
Female
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
E4.5 pregnant
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Specimen
not shown:
postnatal adult
(more )
Note: nonpregnant
Specimen
not shown:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
nonpregnant
|
Specimen
not shown:
postnatal adult
(more )
Note: E5.5 or E6.5 pregnant
Specimen
not shown:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
E5.5 or E6.5 pregnant
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Specimen
not shown:
postnatal adult
(more )
Note: E12.5 pregnant
Specimen
not shown:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal adult
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Sex:
|
Female
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
E12.5 pregnant
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua |
Absent |
|
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After E9.5, expression gradually disappeared and could no longer be detected in the remnants of the deteriorating antimesometrial decidual layer on E12.5.
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