Cdkn1b Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:345409 Cook AG, et al., Cell division angle predicts the level of tissue mechanics that tune the amount of cerebellar folding. Development. 2024 Feb 1;151(3):dev202184
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7641922
Gene symbol:	Cdkn1b
Gene name:	cyclin dependent kinase inhibitor 1B

Antibody:	anti-p27^Kip1 #610241, 610242
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

5M upper ©

Specimen 5M upper: postnatal day 5 (more

)
Note: Double labeled: magenta - Cdkn1b; cyan - EDU (1 h).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell	Present	Regionally restricted	5M upper	Expression is seen in differentiating granule cells.
TS28: cerebellum vermis lobule VI	cerebellar granule cell	Present	Regionally restricted	5M upper	Expression is seen in differentiating granule cells.
TS28: cerebellum vermis lobule VII	cerebellar granule cell	Present	Regionally restricted	5M upper	Expression is seen in differentiating granule cells.

5M lower ©

Specimen 5M lower: postnatal day 5 (more

)
Note: Double labeled: magenta - Cdkn1b; cyan - EDU (24 h).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell	Present	Regionally restricted	5M lower	Expression is seen in differentiating granule cells.
TS28: cerebellum vermis lobule VI	cerebellar granule cell	Present	Regionally restricted	5M lower	Expression is seen in differentiating granule cells.
TS28: cerebellum vermis lobule VII	cerebellar granule cell	Present	Regionally restricted	5M lower	Expression is seen in differentiating granule cells.

Specimen 5Q - Start - C57BL/6J: postnatal day 5 (more

)

Structure	Cell Type	Level	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell	Present	The number of granule cells is reported in Fig. 5Q.
TS28: cerebellum vermis lobule VI	cerebellar granule cell	Present	The number of granule cells is reported in Fig. 5Q.
TS28: cerebellum vermis lobule VII	cerebellar granule cell	Present	The number of granule cells is reported in Fig. 5Q.

Specimen 5Q - Start - FVB/NJ: postnatal day 3 (more

)

Structure	Cell Type	Level	Note
TS27: cerebellum external granule cell layer	cerebellar granule cell	Present	The number of granule cells is reported in Fig. 5Q.
TS27: cerebellum vermis lobule VI	cerebellar granule cell	Present	The number of granule cells is reported in Fig. 5Q.
TS27: cerebellum vermis lobule VII	cerebellar granule cell	Present	The number of granule cells is reported in Fig. 5Q.

Specimen 5Q - End - C57BL/6J: postnatal day 7 (more

)

Structure	Cell Type	Level	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell	Present	The number of granule cells is reported in Fig. 5Q.
TS28: cerebellum vermis lobule VI	cerebellar granule cell	Present	The number of granule cells is reported in Fig. 5Q.
TS28: cerebellum vermis lobule VII	cerebellar granule cell	Present	The number of granule cells is reported in Fig. 5Q.

Specimen 5Q - End- FVB/NJ: postnatal day 5 (more

)

Structure	Cell Type	Level	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell	Present	The number of granule cells is reported in Fig. 5Q.
TS28: cerebellum vermis lobule VI	cerebellar granule cell	Present	The number of granule cells is reported in Fig. 5Q.
TS28: cerebellum vermis lobule VII	cerebellar granule cell	Present	The number of granule cells is reported in Fig. 5Q.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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