Gene Expression Data
Assay Details
Assay
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Reference:
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J:35158
Deman J, et al., In vivo expression of interleukin-1 beta (IL-1 beta), IL-2, IL-4, IL-6, tumour necrosis factor-alpha and interferon-gamma in the fetal murine thymus. Immunology. 1996 Sep;89(1):152-7
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7641600
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Gene symbol:
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Tnf
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Gene name:
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tumor necrosis factor
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Antibody:
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anti-TNFalpha (IP-400)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1e:
embryonic day 19.5
(more )
Specimen
1e:
(close )
Genetic Background:
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C57BL/Ka
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Age:
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embryonic day 19.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: thymus |
stromal cell of thymus |
Present |
Regionally restricted |
1e
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xpression in thymocytes and stromal cells, preferentially in external area of thymus. Expression in both cortex and medulla.
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TS26: thymus |
thymocyte |
Present |
Regionally restricted |
1e
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xpression in thymocytes and stromal cells, preferentially in external area of thymus. Expression in both cortex and medulla.
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Specimen
day 13:
embryonic day 13.5
(more )
Note: Data presented in Figure 3.
Specimen
day 13:
(close )
Genetic Background:
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C57BL/Ka
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Data presented in Figure 3.
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Structure |
Level |
Pattern |
Image |
Note |
TS21: thymus primordium |
Present |
Regionally restricted |
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Weakly expressing cells in external area of thymus on day 13.
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Specimen
day 14:
embryonic day 14.5
(more )
Note: Data presented in Figure 3.
Specimen
day 14:
(close )
Genetic Background:
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C57BL/Ka
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Data presented in Figure 3.
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Specimen
day 15:
embryonic day 15.5
(more )
Note: Data presented in Figure 3.
Specimen
day 15:
(close )
Genetic Background:
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C57BL/Ka
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Age:
|
embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Data presented in Figure 3.
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Specimen
day 16:
embryonic day 16.5
(more )
Note: Data presented in Figure 3.
Specimen
day 16:
(close )
Genetic Background:
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C57BL/Ka
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Data presented in Figure 3.
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Specimen
day 17:
embryonic day 17.5
(more )
Note: Data presented in Figure 3.
Specimen
day 17:
(close )
Genetic Background:
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C57BL/Ka
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Data presented in Figure 3.
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Specimen
day 18:
embryonic day 18.5
(more )
Note: Data presented in Figure 3.
Specimen
day 18:
(close )
Genetic Background:
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C57BL/Ka
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Age:
|
embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Data presented in Figure 3.
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Specimen
day 19:
embryonic day 19.5
(more )
Note: Data presented in Figure 3.
Specimen
day 19:
(close )
Genetic Background:
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C57BL/Ka
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Age:
|
embryonic day 19.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
|
Note:
|
Data presented in Figure 3.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: thymus |
Strong |
Regionally restricted |
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Strong expression in both cortex and medulla on day 19.
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Specimen
day 20:
embryonic day 20.5
(more )
Note: Data presented in Figure 3.
Specimen
day 20:
(close )
Genetic Background:
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C57BL/Ka
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Age:
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embryonic day 20.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Data presented in Figure 3.
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