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Gene Expression Data
Assay Details
Assay
Reference: J:326026 Prokopuk L, et al., An essential role for Polycomb Repressive Complex 2 in the mouse ovary. Reproduction. 2022 Feb 17;163(3):167-182
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7640176
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-phospho-histone H2A.X (Ser139) (CST;#2577)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 3E Eedwt/wt: embryonic day 16.5 (more )
Note: Triple labeled: cyan - H2ax; green - Ddx4 (assay MGI:7640168); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present Regionally restricted 3E Eed wt/wt p-gamma H2AX , 3E Eed wt/wt merge Expression was detected in meiotic germ cells.
Specimen 3E Eedhypo/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: cyan - H2ax; green - Ddx4 (assay MGI:7640168); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present Regionally restricted 3E Eed hypo/hypo p-gamma H2AX , 3E Eed hypo/hypo merge Expression was detected in meiotic germ cells in the mutant at similar levels to wild type.
Specimen S2 Eedwt/wt: embryonic day 16.5 (more )
Note: Triple labeled: cyan - H2ax; green - Ddx4 (assay MGI:7640168); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present Regionally restricted S2_Eed wt/wt merge , S2_Eed wt/wt p-gamma H2AX Expression was detected in meiotic germ cells.
Specimen S2 Eedwt/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eed+ (more )
Note: Triple labeled: cyan - H2ax; green - Ddx4 (assay MGI:7640168); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present Regionally restricted S2_Eed wt/hypo merge , S2_Eed wt/hypo p-gamma H2AX Expression was detected in meiotic germ cells.
Specimen S2 Eedhypo/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: cyan - H2ax; green - Ddx4 (assay MGI:7640168); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present Regionally restricted S2_Eed hypo/hypo p-gamma H2AX , S2_Eed hypo/hypo merge Expression was detected in meiotic germ cells in the mutant at similar levels to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory