Nr2f2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:326026 Prokopuk L, et al., An essential role for Polycomb Repressive Complex 2 in the mouse ovary. Reproduction. 2022 Feb 17;163(3):167-182
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7640170
Gene symbol:	Nr2f2
Gene name:	nuclear receptor subfamily 2, group F, member 2

Antibody:	anti-COUP-TFII (PP-H7147-00)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

Specimen 1B Eed^wt/wt: embryonic day 14.5 (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H3K27me3.

Structure	Level	Pattern	Image	Note
TS22: ovary	Present	Regionally restricted	1B Eed wt/wt Nr2f2 , 1B Eed wt/wt merge	Expression was detected in somatic cells.

Specimen 1B Eed^wt/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H3K27me3.

Structure	Level	Pattern	Image	Note
TS22: ovary	Present	Regionally restricted	1B Eed wt/hypo merge , 1B Eed wt/hypo Nr2f2	Expression was detected in somatic cells.

Specimen 1B Eed^hypo/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H3K27me3.

Structure	Level	Pattern	Image	Note
TS22: ovary	Present	Regionally restricted	1B Eed hypo/hypo merge , 1B Eed hypo/hypo Nr2f2	Expression was detected in somatic cells.

Specimen 3E Eed^wt/wt: embryonic day 16.5 (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H2ax (assay MGI:7640176).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		3E Eed wt/wt merge

Specimen 3E Eed^hypo/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H2ax (assay MGI:7640176).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		3E Eed hypo/hypo merge

Specimen 4A Eed^wt/wt: embryonic day 14.5 (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Foxl2 (assay MGI:7640180).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		4A Eed wt/wt merge , 4A Eed wt/wt Nr2f2

Specimen 4A Eed^wt/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Foxl2 (assay MGI:7640180).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		4A Eed wt/hypo merge , 4A Eed wt/hypo Nr2f2

Specimen 4A Eed^hypo/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Foxl2 (assay MGI:7640180).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		4A Eed hypo/hypo merge , 4A Eed hypo/hypo Nr2f2	Expression in the mutant was similar to wild type.

Specimen 4B Eed^wt/wt: embryonic day 14.5 (more

)
Note: Double labeled: red - Nr2f2; green - Gata4 (assay MGI:7640181).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		4B Eed wt/wt merge , 4B Eed wt/wt Nr2f2

Specimen 4B Eed^wt/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Double labeled: red - Nr2f2; green - Gata4 (assay MGI:7640181).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		4B Eed wt/hypo Nr2f2 , 4B Eed wt/hypo merge

Specimen 4B Eed^hypo/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Double labeled: red - Nr2f2; green - Gata4 (assay MGI:7640181).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		4B Eed hypo/hypo merge , 4B Eed hypo/hypo Nr2f2	Expression in the mutant was similar to wild type.

Specimen S1A Eed^wt/wt: embryonic day 14.5 (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H3K27me3.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S1A Eed wt/wt Nr2f2 , S1A Eed wt/wt merge

Specimen S1A Eed^wt/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H3K27me3.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S1A Eed wt/hypo merge , S1A Eed wt/hypo Nr2f2

Specimen S1A Eed^hypo/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H3K27me3.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S1A Eed hypo/hypo Nr2f2 , S1A Eed hypo/hypo merge

Specimen S1B Eed^wt/wt: embryonic day 16.5 (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H3K27me3.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S1B Eed wt/wt Nr2f2 , S1B Eed wt/wt merge

Specimen S1B Eed^wt/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H3K27me3.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S1B Eed wt/hypo merge , S1B Eed wt/hypo Nr2f2

Specimen S1B Eed^hypo/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H3K27me3.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S1B Eed hypo/hypo merge , S1B Eed hypo/hypo Nr2f2

Specimen S2 Eed^wt/wt: embryonic day 16.5 (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H2ax (assay MGI:7640176).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S2_Eed wt/wt merge , S2_Eed wt/wt Nr2f2

Specimen S2 Eed^wt/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H2ax (assay MGI:7640176).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S2_Eed wt/hypo merge , S2_Eed wt/hypo Nr2f2

Specimen S2 Eed^hypo/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - H2ax (assay MGI:7640176).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S2_Eed hypo/hypo merge , S2_Eed hypo/hypo Nr2f2

Specimen S3A Eed^wt/wt: embryonic day 14.5 (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Foxl2 (assay MGI:7640180).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S3A Eed wt/wt merge , S3A Eed wt/wt Nr2f2

Specimen S3A Eed^wt/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Foxl2 (assay MGI:7640180).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S3A Eed wt/hypo merge , S3A Eed wt/hypo Nr2f2

Specimen S3A Eed^hypo/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Foxl2 (assay MGI:7640180).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S3A Eed hypo/hypo Nr2f2 , S3A Eed hypo/hypo merge

Specimen S3B Eed^wt/wt: embryonic day 16.5 (more

)
Note: Double labeled: red - Nr2f2; cyan - Foxl2 (assay MGI:7640180).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S3B Eed wt/wt Nr2f2 , S3B Eed wt/wt merge

Specimen S3B Eed^wt/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Double labeled: red - Nr2f2; cyan - Foxl2 (assay MGI:7640180).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S3B Eed wt/hypo Nr2f2 , S3B Eed wt/hypo merge

Specimen S3B Eed^hypo/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Double labeled: red - Nr2f2; cyan - Foxl2 (assay MGI:7640180).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S3B Eed hypo/hypo Nr2f2 , S3B Eed hypo/hypo merge

Specimen S4 Eed^wt/wt: embryonic day 14.5 (more

)
Note: Double labeled: red - Nr2f2; green - Gata4 (assay MGI:7640181).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S4_Eed wt/wt merge , S4_Eed wt/wt Nr2f2

Specimen S4 Eed^wt/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Double labeled: red - Nr2f2; green - Gata4 (assay MGI:7640181).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S4_Eed wt/hypo merge , S4_Eed wt/hypo Nr2f2

Specimen S4 Eed^hypo/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Double labeled: red - Nr2f2; green - Gata4 (assay MGI:7640181).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S4_Eed hypo/hypo Nr2f2 , S4_Eed hypo/hypo merge

Specimen S5A Eed^wt/wt: embryonic day 14.5 (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Laminin.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S5A Eed wt/wt Nr2f2 , S5A Eed wt/wt merge

Specimen S5A Eed^wt/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Laminin.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S5A Eed wt/hypo Nr2f2 , S5A Eed wt/hypo merge

Specimen S5A Eed^hypo/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Laminin.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S5A Eed hypo/hypo Nr2f2 , S5A Eed hypo/hypo merge

Specimen S5B Eed^wt/wt: embryonic day 16.5 (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Laminin.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S5B Eed wt/wt Nr2f2 , S5B Eed wt/wt merge

Specimen S5B Eed^wt/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Laminin.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S5B Eed wt/hypo merge , S5B Eed wt/hypo Nr2f2

Specimen S5B Eed^hypo/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Laminin.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S5B Eed hypo/hypo merge , S5B Eed hypo/hypo Nr2f2

Specimen S6A Eed^wt/wt: embryonic day 14.5 (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Mki67 (assay MGI:7641416).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S6A Eed wt/wt Nr2f2 , S6A Eed wt/wt merge

Specimen S6A Eed^wt/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Mki67 (assay MGI:7641416).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S6A Eed wt/hypo merge , S6A Eed wt/hypo Nr2f2

Specimen S6A Eed^hypo/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Mki67 (assay MGI:7641416).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S6A Eed hypo/hypo merge , S6A Eed hypo/hypo Nr2f2

Specimen S6B Eed^wt/wt: embryonic day 16.5 (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Mki67 (assay MGI:7641416).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S6B Eed wt/wt Nr2f2 , S6B Eed wt/wt merge

Specimen S6B Eed^wt/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Mki67 (assay MGI:7641416).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S6B Eed wt/hypo merge , S6B Eed wt/hypo Nr2f2

Specimen S6B Eed^hypo/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Mki67 (assay MGI:7641416).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S6B Eed hypo/hypo merge , S6B Eed hypo/hypo Nr2f2

Specimen S8A Eed^wt/wt: embryonic day 14.5 (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Hsd3b (assay MGI:7641582).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S8A Eed wt/wt merge , S8A Eed wt/wt Nr2f2

Specimen S8A Eed^wt/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Hsd3b (assay MGI:7641582).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S8A Eed wt/hypo merge , S8A Eed wt/hypo Nr2f2

Specimen S8A Eed^hypo/hypo: embryonic day 14.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Triple labeled: red - Nr2f2; green - Ddx4 (assay MGI:7640168); cyan - Hsd3b (assay MGI:7641582).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: ovary	interstitial cell of ovary	Present		S8A Eed hypo/hypo Nr2f2 , S8A Eed hypo/hypo merge

Specimen S8B Eed^wt/wt: embryonic day 16.5 (more

)
Note: Double labeled: red - Nr2f2; green - Hsd3b (assay MGI:7641583).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S8B Eed wt/wt merge , S8B Eed wt/wt Nr2f2

Specimen S8B Eed^wt/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed⁺ (more

)
Note: Double labeled: red - Nr2f2; green - Hsd3b (assay MGI:7641583).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S8B Eed wt/hypo merge , S8B Eed wt/hypo Nr2f2

Specimen S8B Eed^hypo/hypo: embryonic day 16.5; Eed^l7Rn5-1989SB/Eed^l7Rn5-1989SB (more

)
Note: Double labeled: red - Nr2f2; green - Hsd3b (assay MGI:7641583).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ovary	interstitial cell of ovary	Present		S8B Eed hypo/hypo Nr2f2 , S8B Eed hypo/hypo merge

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