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Gene Expression Data
Assay Details
Assay
Reference: J:326026 Prokopuk L, et al., An essential role for Polycomb Repressive Complex 2 in the mouse ovary. Reproduction. 2022 Feb 17;163(3):167-182
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7640168
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: Anti-DDX4 (D10C5) #8761
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: There were two antibodies described MGI:7628718 and MGI:6724501; the one used in this assay was not specified.
Results
Specimen 1B Eedwt/wt: embryonic day 14.5 (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - H3K27me3.
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 1B Eed wt/wt MVH , 1B Eed wt/wt merge
Specimen 1B Eedwt/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eed+ (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - H3K27me3.
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 1B Eed wt/hypo MVH , 1B Eed wt/hypo merge
Specimen 1B Eedhypo/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - H3K27me3.
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 1B Eed hypo/hypo MVH , 1B Eed hypo/hypo merge
Specimen 3A Eedwt/wt left: embryonic day 14.5 (more )
Note: Double labeled: green - Ddx4; red - Stra8 (assay MGI:7640174).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 3A Eed wt/wt MVH/Stra8
Specimen 3A Eedhypo/hypo left: embryonic day 14.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Double labeled: green - Ddx4; red - Stra8 (assay MGI:7640174).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 3A Eed hypo/hypo MVH/Stra8
Specimen 3A Eedwt/wt right: embryonic day 14.5 (more )
Note: Double labeled: green - Ddx4; red - Sycp3 (assay MGI:7640175).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 3A Eed wt/wt MVH/Sycp3
Specimen 3A Eedhypo/hypo right: embryonic day 14.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Double labeled: green - Ddx4; red - Sycp3 (assay MGI:7640175).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 3A Eed hypo/hypo MVH/Sycp3
Specimen 3E Eedwt/wt: embryonic day 16.5 (more )
Note: Triple labeled: green - Ddx4; cyan - H2ax (assay MGI:7640176); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present 3E Eed wt/wt MVH , 3E Eed wt/wt merge
Specimen 3E Eedhypo/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: green - Ddx4; cyan - H2ax (assay MGI:7640176); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present 3E Eed hypo/hypo merge , 3E Eed hypo/hypo MVH
Specimen 4A Eedwt/wt: embryonic day 14.5 (more )
Note: Triple labeled: green - Ddx4; cyan - Foxl2 (assay MGI:7640180); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 4A Eed wt/wt merge , 4A Eed wt/wt MVH
Specimen 4A Eedwt/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eed+ (more )
Note: Triple labeled: green - Ddx4; cyan - Foxl2 (assay MGI:7640180); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 4A Eed wt/hypo MVH , 4A Eed wt/hypo merge
Specimen 4A Eedhypo/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: green - Ddx4; cyan - Foxl2 (assay MGI:7640180); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 4A Eed hypo/hypo merge , 4A Eed hypo/hypo MVH
Specimen 7B Eedwt/wt: embryonic day 16.5 (more )
Note: Double labeled: green - Ddx4; red - Sox9 (assay MGI:7641388).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present 7B Eed wt/wt merge
Specimen 7B Eedhypo/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Double labeled: green - Ddx4; red - Sox9 (assay MGI:7641388).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present 7B Eed hypo/hypo merge
Specimen 7B XY control: embryonic day 16.5 (more )
Note: Double labeled: green - Ddx4; red - Sox9 (assay MGI:7641388).
Structure Level Pattern Image Note
TS24: germ cell of testis Present 7B XY control merge
Specimen S1A Eedwt/wt: embryonic day 14.5 (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - H3K27me3.
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S1A Eed wt/wt MVH , S1A Eed wt/wt merge
Specimen S1A Eedwt/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eed+ (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - H3K27me3.
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S1A Eed wt/hypo MVH , S1A Eed wt/hypo merge
Specimen S1A Eedhypo/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - H3K27me3.
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S1A Eed hypo/hypo MVH , S1A Eed hypo/hypo merge
Specimen S1B Eedwt/wt: embryonic day 16.5 (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - H3K27me3.
Structure Level Pattern Image Note
TS24: germ cell of ovary Present S1B Eed wt/wt MVH , S1B Eed wt/wt merge
Specimen S1B Eedwt/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eed+ (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - H3K27me3.
Structure Level Pattern Image Note
TS24: germ cell of ovary Present S1B Eed wt/hypo merge , S1B Eed wt/hypo MVH
Specimen S1B Eedhypo/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - H3K27me3.
Structure Level Pattern Image Note
TS24: germ cell of ovary Present S1B Eed hypo/hypo merge , S1B Eed hypo/hypo MVH
Specimen S2 Eedwt/wt: embryonic day 16.5 (more )
Note: Triple labeled: green - Ddx4; cyan - H2ax (assay MGI:7640176); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present S2_Eed wt/wt merge , S2_Eed wt/wt MVH
Specimen S2 Eedwt/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eed+ (more )
Note: Triple labeled: green - Ddx4; cyan - H2ax (assay MGI:7640176); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present S2_Eed wt/hypo MVH , S2_Eed wt/hypo merge
Specimen S2 Eedhypo/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: green - Ddx4; cyan - H2ax (assay MGI:7640176); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present S2_Eed hypo/hypo MVH , S2_Eed hypo/hypo merge
Specimen S3A Eedwt/wt: embryonic day 14.5 (more )
Note: Triple labeled: green - Ddx4; cyan - Foxl2 (assay MGI:7640180); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S3A Eed wt/wt MVH , S3A Eed wt/wt merge
Specimen S3A Eedwt/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eed+ (more )
Note: Triple labeled: green - Ddx4; cyan - Foxl2 (assay MGI:7640180); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S3A Eed wt/hypo MVH , S3A Eed wt/hypo merge
Specimen S3A Eedhypo/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: green - Ddx4; cyan - Foxl2 (assay MGI:7640180); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S3A Eed hypo/hypo merge , S3A Eed hypo/hypo MVH
Specimen S5A Eedwt/wt: embryonic day 14.5 (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - Laminin.
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S5A Eed wt/wt merge , S5A Eed wt/wt MVH
Specimen S5A Eedwt/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eed+ (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - Laminin.
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S5A Eed wt/hypo MVH , S5A Eed wt/hypo merge
Specimen S5A Eedhypo/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - Laminin.
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S5A Eed hypo/hypo MVH , S5A Eed hypo/hypo merge
Specimen S5B Eedwt/wt: embryonic day 16.5 (more )
Note: Double labeled: green - Ddx4; cyan - Laminin.
Structure Level Pattern Image Note
TS24: germ cell of ovary Present S5B Eed wt/wt merge , S5B Eed wt/wt MVH
Specimen S5B Eedwt/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eed+ (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - Laminin.
Structure Level Pattern Image Note
TS24: germ cell of ovary Present S5B Eed wt/hypo merge , S5B Eed wt/hypo MVH
Specimen S5B Eedhypo/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: green - Ddx4; red - Nr2f2 (assay MGI:7640170); cyan - Laminin.
Structure Level Pattern Image Note
TS24: germ cell of ovary Present S5B Eed hypo/hypo MVH , S5B Eed hypo/hypo merge
Specimen S6A Eedwt/wt: embryonic day 14.5 (more )
Note: Triple labeled: green - Ddx4; cyan - Mki67 (assay MGI:7641416); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S6A Eed wt/wt merge , S6A Eed wt/wt MVH
Specimen S6A Eedwt/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eed+ (more )
Note: Triple labeled: green - Ddx4; cyan - Mki67 (assay MGI:7641416); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S6A Eed wt/hypo merge , S6A Eed wt/hypo MVH
Specimen S6A Eedhypo/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: green - Ddx4; cyan - Mki67 (assay MGI:7641416); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S6A Eed hypo/hypo MVH , S6A Eed hypo/hypo merge
Specimen S6B Eedwt/wt: embryonic day 16.5 (more )
Note: Triple labeled: green - Ddx4; cyan - Mki67 (assay MGI:7641416); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present S6B Eed wt/wt MVH , S6B Eed wt/wt merge
Specimen S6B Eedwt/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eed+ (more )
Note: Triple labeled: green - Ddx4; cyan - Mki67 (assay MGI:7641416); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present S6B Eed wt/hypo MVH , S6B Eed wt/hypo merge
Specimen S6B Eedhypo/hypo: embryonic day 16.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: green - Ddx4; cyan - Mki67 (assay MGI:7641416); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS24: germ cell of ovary Present S6B Eed hypo/hypo merge , S6B Eed hypo/hypo MVH
Specimen S8A Eedwt/wt: embryonic day 14.5 (more )
Note: Triple labeled: green - Ddx4; cyan - Hsd3b (assay MGI:7641582); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S8A Eed wt/wt MVH , S8A Eed wt/wt merge
Specimen S8A Eedwt/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eed+ (more )
Note: Triple labeled: green - Ddx4; cyan - Hsd3b (assay MGI:7641582); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S8A Eed wt/hypo MVH , S8A Eed wt/hypo merge
Specimen S8A Eedhypo/hypo: embryonic day 14.5; Eedl7Rn5-1989SB/Eedl7Rn5-1989SB (more )
Note: Triple labeled: green - Ddx4; cyan - Hsd3b (assay MGI:7641582); red - Nr2f2 (assay MGI:7640170).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S8A Eed hypo/hypo merge , S8A Eed hypo/hypo MVH
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