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Gene Expression Data
Assay Details
Assay
Reference: J:327416 Pailles M, et al., H3K27me3 at pericentromeric heterochromatin is a defining feature of the early mouse blastocyst. Sci Rep. 2022 Aug 16;12(1):13908
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7639750
Gene symbol: Bend3
Gene name: BEN domain containing 3
Antibody: anti-BEND3
Results
Specimen 5e 2-cell: embryonic day 1.0 (more )
Note: Double labeled: red - Bend3; green - DAPI.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Absent 5e 2-cell BEND3 , 5e 2-cell (merged)
Specimen 5e 4-cell: embryonic day 2.0 (more )
Note: Double labeled: red - Bend3; green - DAPI.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent 5e 4-cell BEND3 , 5e 4-cell (merged)
Specimen 5e 8-cell: embryonic day 2.5 (more )
Note: Double labeled: red - Bend3; green - DAPI.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted 5e 8-cell BEND3 , 5e 8-cell (merged) Expression enriched at pericentromeric heterochromatin starting at 8-cell stage.
Specimen 5e 16-cell: embryonic day 3.0 (more )
Note: Double labeled: red - Bend3; green - DAPI.
Structure Level Pattern Image Note
TS3: compacted morula Present 5e 16-cell (merged) , 5e 16-cell BEND3
Specimen 5g E3.5: embryonic day 3.5 (more )
Note: Double labeled: red - Bend3; green - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Present 5g E3.5 (merged) , 5g E3.5 BEND3
Specimen 5g E4.0: embryonic day 4.0 (more )
Note: Double labeled: red - Bend3; green - DAPI.
Structure Level Pattern Image Note
TS6: blastocyst Present 5g E4.0 (merged) , 5g E4.0 BEND3 Expression conserved up to E5.0 in all cells.
Specimen 5g E5.5: embryonic day 5.5 (more )
Note: Double labeled: red - Bend3; green - DAPI.
Structure Level Pattern Image Note
TS7: egg cylinder Absent 5g E5.5 (merged) , 5g E5.5 BEND3 Expression lost at E5.5.
Specimen S5a BEND3: embryonic day 3.5 (more )
Note: Double labeled: red - Bend3; green - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Present S5a BEND3
Specimen S5b BEND3: embryonic day 4.0 (more )
Note: Double labeled: red - Bend3; green - DAPI.
Structure Level Pattern Image Note
TS6: blastocyst Present S5b BEND3 Expression conserved up to E5.0 in all cells.
Specimen S5c BEND3: embryonic day 5.5 (more )
Note: Double labeled: red - Bend3; green - DAPI.
Structure Level Pattern Image Note
TS7: egg cylinder Absent S5c BEND3 Expression lost at E5.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory