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Gene Expression Data
Assay Details
Assay
Reference: J:327416 Pailles M, et al., H3K27me3 at pericentromeric heterochromatin is a defining feature of the early mouse blastocyst. Sci Rep. 2022 Aug 16;12(1):13908
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7639745
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: anti-NANOG
Results
Specimen 2a NANOG: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 2a NANOG Expression in epiblast precursors within inner cell mass.
Specimen 2c NANOG (upper): embryonic day 4.0 (more )
Note: Double labeled: green - Nanog; white - Pou5f1 (assay MGI:7639747).
Structure Level Pattern Image Note
TS6: epiblast Present 2c OCT4/NANOG (upper) Expression restricted to pluripotent epiblast.
Specimen 2c NANOG (lower): embryonic day 4.0 (more )
Note: Double labeled: green - Nanog; white - Pou5f1 (assay MGI:7639747).
Structure Level Pattern Image Note
TS6: epiblast Present 2c OCT4/NANOG (lower) Expression restricted to pluripotent epiblast.
Specimen S2b NANOG: embryonic day 3.25 (more )
Note: Authors report blastocyst stages to be at E3.25 to E4.25. Double labeled: green - Nanog; white - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Present S2b NANOG
Specimen S2c NANOG: embryonic day 3.5 (more )
Note: Double labeled: green - Nanog; white - DAPI.
Structure Level Pattern Image Note
TS4: inner cell mass Present S2c NANOG Expression in epiblast precursors within inner cell mass.
Specimen S2e NANOG: embryonic day 3.75 (more )
Note: Double labeled: green - Nanog; magenta - Gata6 (assay MGI:7639748).
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted S2e NANOG/GATA6 Authors report spatial segregation of epiblast and primitive endoderm precursors within inner cell mass.
Specimen S2f NANOG: embryonic day 4.0 (more )
Note: Double labeled: green - Nanog; magenta - Gata6 (assay MGI:7639748).
Structure Level Pattern Image Note
TS6: epiblast Present S2f NANOG/GATA6 Expression restricted to pluripotent epiblast.
Specimen S2h NANOG: embryonic day 4.25 (more )
Note: Authors report blastocyst stages to be at E3.25 to E4.25, with implantation at E4.25. Double labeled: green - Nanog; magenta - Gata6 (assay MGI:7639748).
Structure Level Pattern Image Note
TS6: blastocyst Present S2h NANOG/GATA6
Specimen 4a NANOG: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS4: inner cell mass Present 4a NANOG Expression in pluripotent cells.
Specimen 4b NANOG: embryonic day 4.0 (more )
Structure Level Pattern Image Note
TS6: epiblast Present 4b NANOG Expression in pluripotent cells.
Specimen S5a NANOG: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS4: inner cell mass Present S5a NANOG Expression in pluripotent cells.
Specimen S5b NANOG: embryonic day 4.0 (more )
Structure Level Pattern Image Note
TS6: epiblast Present S5b NANOG Expression in pluripotent cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory