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Gene Expression Data
Assay Details
Assay
Reference: J:347711 Morikawa M, et al., Mesenchymal cells regulate enteric neural crest cell migration via RET-GFRA1b trans-signaling. Biochem Biophys Res Commun. 2024 May 28;710:149861
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7638764
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1E E12.0: embryonic day 12.0 (more )
Note: Double labeled: red - Acta2; green - Gfra1 (assay MGI:7638765).
Structure Level Pattern Image Note
TS20: gut Present Regionally restricted 1E E12.0 After E12.0, expression gradually increased in area between serosa membrane and intestinal lumen.
Specimen 1E E12.5: embryonic day 12.5 (more )
Note: Double labeled: red - Acta2; green - Gfra1 (assay MGI:7638765).
Structure Level Pattern Image Note
TS20: gut Present 1E E12.5
Specimen 1E E13.0: embryonic day 13.0 (more )
Note: Double labeled: red - Acta2; green - Gfra1 (assay MGI:7638765).
Structure Level Pattern Image Note
TS21: gut Present 1E E13.0
Specimen 1E E13.5: embryonic day 13.5 (more )
Note: Double labeled: red - Acta2; green - Gfra1 (assay MGI:7638765).
Structure Level Pattern Image Note
TS21: gut Strong Regionally restricted 1E E13.5 After E13.5, expression intensifies, esprecially on intestinal lumen side, and around enteric nerve.
Specimen S1 E11.5 row3: embryonic day 11.5 (more )
Note: Double labeled: green - Acta2; red - Uchl1 (assay MGI:7638767).
Structure Level Pattern Image Note
TS19: gut Absent S1_E11.5 row3 No expression in intestinal tract at E11.5
Specimen S1 E12.5 MG row3: embryonic day 12.5 (more )
Note: Double labeled: green - Acta2; red - Uchl1 (assay MGI:7638767).
Structure Level Pattern Image Note
TS20: hindgut Present Widespread S1_E12.5 MG row3 Expression throughout intestinal tract begins at E12.5.
Specimen S1 E12.5 HG row3: embryonic day 12.5 (more )
Note: Double labeled: green - Acta2; red - Uchl1 (assay MGI:7638767).
Structure Level Pattern Image Note
TS20: hindgut Present Widespread S1_E12.5 HG row3 Expression throughout intestinal tract begins at E12.5.
Specimen S1 E15.5 MG row3: embryonic day 15.5 (more )
Note: Double labeled: green - Acta2; red - Uchl1 (assay MGI:7638767).
Structure Level Pattern Image Note
TS23: midgut Strong Regionally restricted S1_E15.5 MG row3 After E13.5, expression intensifies, esprecially on intestinal lumen side, and around enteric nerve.
Specimen S1 E15.5 HG row3: embryonic day 15.5 (more )
Note: Double labeled: green - Acta2; red - Uchl1 (assay MGI:7638767).
Structure Level Pattern Image Note
TS23: hindgut Strong Regionally restricted S1_E15.5 HG row3 After E13.5, expression intensifies, esprecially on intestinal lumen side, and around enteric nerve.
Specimen S2A E11.5: embryonic day 11.5 (more )
Note: Triple labeled: green - Acta2; red - Gfra1 (assay MGI:7638765); blue - Tubb3 (assay MGI:7638763).
Structure Level Pattern Image Note
TS19: midgut Absent S2A E11.5 (upper) , S2A E11.5 (lower) No expression in intestinal tract at E11.5
Specimen S2A E12.0, S2B: embryonic day 12.0 (more )
Note: Triple labeled: green - Acta2; red - Gfra1 (assay MGI:7638765); blue - Tubb3 (assay MGI:7638763).
Structure Level Pattern Image Note
TS20: midgut Present Regionally restricted S2A E12.0 (upper) , S2B Merge , S2A E12.0 (lower) , S2B SMA/TuJ1 Expression gradually increased in area between serosa membrane and intestinal lumen after E12.0. Strong co-localization of expression with Gfra1 in luminal region of Gfra1-expressing ring of cells.
Specimen S2A E12.5: embryonic day 12.5 (more )
Note: Triple labeled: green - Acta2; red - Gfra1 (assay MGI:7638765); blue - Tubb3 (assay MGI:7638763).
Structure Level Pattern Image Note
TS20: midgut Present Widespread S2A E12.5 (upper) , S2A E12.5 (lower) Expression throughout intestinal tract begins at E12.5.
Specimen S2A E13.0: embryonic day 13.0 (more )
Note: Triple labeled: green - Acta2; red - Gfra1 (assay MGI:7638765); blue - Tubb3 (assay MGI:7638763).
Structure Level Pattern Image Note
TS21: midgut Present Widespread S2A E13.0 (lower) , S2A E13.0 (upper) Expression throughout intestinal tract begins at E12.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory