Foxl2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:346442 Migale R, et al., FOXL2 interaction with different binding partners regulates the dynamics of ovarian development. Sci Adv. 2024 Mar 22;10(12):eadl0788
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7628312
Gene symbol:	Foxl2
Gene name:	forkhead box L2

Antibody:	Anti-FOXL2 antibody (ab5096)
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Two Foxl2 antibodies were described, but the one used in this assay was not detailed. Anti-FOXL2 MGI:7628290 may have been used in this assay.

Results

S1A XX ovary wildtype ©

Specimen S1A XX ovary wild type: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: ovary	Present		S1A XX ovary wildtype	Expression specific to wildtype ovary.

S1A XX Foxl2cko gonad ©

Specimen S1A XX Foxl2cko gonad: postnatal adult; Foxl2^tm2.1Tre/Foxl2^tm2.1Tre, Tg(Cga-cre)3Sac/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: gonad	Absent		S1A XX Foxl2cko gonad

S1A XY testis wildtype ©

Specimen S1A XY testis wildtype: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: testis	Absent		S1A XY testis wildtype

3B E14.5 XY (lower) ©

Specimen 3B E14.5 XY (lower): embryonic day 14.5; Foxl2^em1Rlb/Foxl2⁺ (more

)
Note: Double labeled: red - Foxl2 protein; green - Foxl2 reporter (assay MGI:7628309).

Structure	Level	Pattern	Image	Note
TS22: testis	Absent		3B E14.5 XY (lower)

3B E14.5 XX (lower) ©

Specimen 3B E14.5 XX (lower): embryonic day 14.5; Foxl2^em1Rlb/Foxl2⁺ (more

)
Note: Double labeled: red - Foxl2 protein; green - Foxl2 reporter (assay MGI:7628309).

Structure	Level	Pattern	Image	Note
TS22: ovary	Present	Regionally restricted	3B E14.5 XX (lower)	Expression colocalizes with that of Foxl2 reporter.

3B 1W XX (lower) ©

Specimen 3B 1W XX (lower): postnatal week 1; Foxl2^em1Rlb/Foxl2⁺ (more

)
Note: Double labeled: red - Foxl2 protein; green - Foxl2 reporter (assay MGI:7628309).

Structure	Level	Pattern	Image	Note
TS28: ovary	Present	Regionally restricted	3B 1W XX (lower)	Expression colocalizes with that of Foxl2 reporter.

3B 8W XX (lower) ©

Specimen 3B 8W XX (lower): postnatal week 8; Foxl2^em1Rlb/Foxl2⁺ (more

)
Note: Double labeled: red - Foxl2 protein; green - Foxl2 reporter (assay MGI:7628309).

Structure	Level	Pattern	Image	Note
TS28: ovary	Present	Regionally restricted	3B 8W XX (lower)	Expression colocalizes with that of Foxl2 reporter.

S8_E14.5 XX EGFP/+ FOXL2 ©

S8_E14.5 XX EGFP/+ GFP-FOXL2 ©

Specimen S8 E14.5 XX: embryonic day 14.5; Foxl2^em1Rlb/Foxl2⁺ (more

)
Note: Double labeled: red - Foxl2 protein; green - Foxl2 reporter (assay MGI:7628309).

Structure	Level	Pattern	Image	Note
TS22: ovary	Present	Regionally restricted	S8_E14.5 XX EGFP/+ GFP-FOXL2 , S8_E14.5 XX EGFP/+ FOXL2	Expression colocalizes with that of Foxl2 reporter.

S8_1W XX EGFP/+ FOXL2 ©

S8_1W XX EGFP/+ GFP-FOXL2 ©

Specimen S8 1W XX: postnatal week 1; Foxl2^em1Rlb/Foxl2⁺ (more

)
Note: Double labeled: red - Foxl2 protein; green - Foxl2 reporter (assay MGI:7628309).

Structure	Level	Pattern	Image	Note
TS28: ovary	Present	Regionally restricted	S8_1W XX EGFP/+ GFP-FOXL2 , S8_1W XX EGFP/+ FOXL2	Expression colocalizes with that of Foxl2 reporter.

S8_8W XX EGFP/+ FOXL2 ©

S8_8W XX EGFP/+ GFP-FOXL2 ©

Specimen S8 8W XX EGFP/+: postnatal week 8; Foxl2^em1Rlb/Foxl2⁺ (more

)
Note: Double labeled: red - Foxl2 protein; green - Foxl2 reporter (assay MGI:7628309).

Structure	Level	Pattern	Image	Note
TS28: ovary	Present	Regionally restricted	S8_8W XX EGFP/+ FOXL2 , S8_8W XX EGFP/+ GFP-FOXL2	Expression colocalizes with that of Foxl2 reporter.

S8_8W XX +/+ FOXL2 ©

S8_8W XX +/+ GFP-FOXL2 ©

Specimen S8 8W XX +/+: postnatal week 8 (more

)

Structure	Level	Pattern	Image	Note
TS28: ovary	Present	Regionally restricted	S8_8W XX +/+ FOXL2 , S8_8W XX +/+ GFP-FOXL2	Expression colocalizes with that of Foxl2 reporter.

S10D (upper) ©

Specimen S10D (upper): postnatal week 8; Foxl2^em1Rlb/Foxl2⁺ (more

)
Note: Double labeled: green - Foxl2; red - Hsd3b (assay MGI:7628317).

Structure	Level	Pattern	Image	Note
TS28: ovary follicle cell	Present		S10D (upper)

5A DDX4-FOXL2 Control ©

Specimen 5A Control: postnatal day 0; Usp7^tm2Wgu/Usp7^tm2Wgu (more

)
Note: Double labeled: red - Foxl2; green - Ddx4 (assay MGI:7628313).

Structure	Level	Pattern	Image	Note
TS27: ovary follicle cell	Present	Regionally restricted	5A DDX4-FOXL2 Control	Expression in granulosa cells organizing into follicles.

5A DDX4-FOXL2 Sf1:Cretg/+;Usp7fl/fl ©

Specimen 5A Sf1:Cretg/+;Usp7fl/fl: postnatal day 0; Tg(Nr5a1-cre)2Klp/0, Usp7^tm2Wgu/Usp7^tm2Wgu (more

)
Note: Conditional mutant. Double labeled: red - Foxl2; green - Ddx4 (assay MGI:7628313).

Structure	Level	Pattern	Image	Note
TS27: ovary follicle cell	Present	Regionally restricted	5A DDX4-FOXL2 Sf1:Cretg/+;Usp7fl/fl	Expression in granulosa cells appearing disorganized in spatial organization.

Specimen 5B Control: postnatal week 1; Usp7^tm2Wgu/Usp7^tm2Wgu (more

)
Note: Double labeled: red - Foxl2; green - Ddx4 (assay MGI:7628313).

Structure	Level	Pattern	Image	Note
TS27: follicle cell of primordial follicle	Present		5B DDX4-FOXL2 Control
TS27: follicle cell of primary follicle	Strong		5B DDX4-FOXL2 Control

Specimen 5B Sf1:Cretg/+;Usp7fl/fl: postnatal week 1; Tg(Nr5a1-cre)2Klp/0, Usp7^tm2Wgu/Usp7^tm2Wgu (more

)
Note: Conditional mutant. Double labeled: red - Foxl2; green - Ddx4 (assay MGI:7628313).

Structure	Level	Pattern	Image	Note
TS28: follicle cell of primordial follicle	Present		5B DDX4-FOXL2 Sf1:Cretg/+;Usp7fl/fl	Expression in granulosa cells only in primordial follicles.
TS28: ovary follicle cell	Present	Regionally restricted	5B DDX4-FOXL2 Sf1:Cretg/+;Usp7fl/fl	Expression in granulosa cells encapsulating nestlike clusters of germ cells.

Specimen S15B Control: postnatal day 0; Usp7^tm2Wgu/Usp7^tm2Wgu (more

)
Note: Double labeled: green - Foxl2; red - Usp7 (assay MGI:7628319).

Structure	Level	Pattern	Image	Note
TS27: ovary follicle cell	Present	Regionally restricted	S15B Control FOXL2 , S15B Control FOXL2/USP7	Co-localization of expression with Usp7 in granulosa cells

Specimen S15B Sf1:Cretg/+;Usp7fl/fl: postnatal day 0; Tg(Nr5a1-cre)2Klp/0, Usp7^tm2Wgu/Usp7^tm2Wgu (more

)
Note: Conditional mutant. Double labeled: green - Foxl2; red - Usp7 (assay MGI:7628319).

Structure	Level	Pattern	Image	Note
TS27: follicle cell of primordial follicle	Present	Regionally restricted	S15B Sf1:Cretg/+;Usp7fl/fl FOXL2 , S15B Sf1:Cretg/+;Usp7fl/fl FOXL2/USP7	Co-localization of expression with Usp7 in granulosa cells

Specimen S15C Control: postnatal day 7; Usp7^tm2Wgu/Usp7^tm2Wgu (more

)
Note: Double labeled: green - Foxl2; red - Usp7 (assay MGI:7628319).

Structure	Level	Pattern	Image	Note
TS28: ovary follicle cell	Present	Regionally restricted	S15C Control FOXL2/USP7 , S15C Control FOXL2	Co-localization of expression with Usp7 in granulosa cells

Specimen S15C Sf1:Cretg/+;Usp7fl/fl: postnatal day 7; Tg(Nr5a1-cre)2Klp/0, Usp7^tm2Wgu/Usp7^tm2Wgu (more

)
Note: Conditional mutant. Double labeled: green - Foxl2; red - Usp7 (assay MGI:7628319).

Structure	Level	Pattern	Image	Note
TS28: follicle cell of primordial follicle	Present	Regionally restricted	S15C Sf1:Cretg/+;Usp7fl/fl FOXL2/USP7 , S15C Sf1:Cretg/+;Usp7fl/fl FOXL2	Co-localization of expression with Usp7 in granulosa cells arrested in primordial follicular stage.

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