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Gene Expression Data
Assay Details
Assay
Reference: J:236179 Bowman CE, et al., Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series. Mol Cell Biol. 2016 Aug 1;36(15):2089-104
Assay type: RT-PCR
MGI Accession ID: MGI:7628296
Gene symbol: Mpc2
Gene name: mitochondrial pyruvate carrier 2
Probe: Mpc2-pF, Mpc2-pR
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to 4 reference genes (18S rRNA, Rpl22, beta-actin, and Gapdh) and shown relative to wild type.
Results Image: 4B Mpc2
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
Cortex WT E17.5 TS25: cerebral cortex Present 0.01 µg; total RNA involves: C57BL/6 * SJL Not Specified
Cortex KI/+ E17.5 TS25: cerebral cortex Present 0.01 µg; total RNA involves: C57BL/6 * SJL Mpc1tm1.1Mwol/Mpc1+Not Specified
Cortex KI/KI E17.5 TS25: cerebral cortex Present (a) 0.01 µg; total RNA involves: C57BL/6 * SJL Mpc1tm1.1Mwol/Mpc1tm1.1MwolNot Specified
Heart WT E17.5 TS25: heart Present 0.01 µg; total RNA involves: C57BL/6 * SJL Not Specified
Heart KI/+ E17.5 TS25: heart Present 0.01 µg; total RNA involves: C57BL/6 * SJL Mpc1tm1.1Mwol/Mpc1+Not Specified
Heart KI/KI E17.5 TS25: heart Present (a) 0.01 µg; total RNA involves: C57BL/6 * SJL Mpc1tm1.1Mwol/Mpc1tm1.1MwolNot Specified
Liver WT E17.5 TS25: liver Present 0.01 µg; total RNA involves: C57BL/6 * SJL Not Specified
Liver KI/+ E17.5 TS25: liver Present 0.01 µg; total RNA involves: C57BL/6 * SJL Mpc1tm1.1Mwol/Mpc1+Not Specified
Liver KI/KI E17.5 TS25: liver Present (a) 0.01 µg; total RNA involves: C57BL/6 * SJL Mpc1tm1.1Mwol/Mpc1tm1.1MwolNot Specified
Notes:
(a) No loss of expression was observed in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory