Antibody:	mAb 25H11
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS17: telencephalon mantle layer	Present		6A
TS17: telencephalon ventricular layer	Present		6A

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present		6C	Immunoreactivity along the lateral plasma membrane of neuroepithelial cells consistently showed a ventricular-high, pial-low gradient.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Absent		8_E9.5

Structure	Level	Pattern	Image	Note
TS17: telencephalon mantle layer	Strong		8_E10.5
TS17: telencephalon ventricular layer	Strong		8_E10.5

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex mantle layer	Strong		8_E12.5
TS20: cerebral cortex ventricular layer	Strong		8_E12.5

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Strong		8_E14.5
TS22: cerebral cortex ventricular layer	Strong		8_E14.5
TS22: cerebral cortex marginal layer	Trace		8_E14.5
TS22: cortical plate	Absent		8_E14.5

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Present		8_E16.5
TS24: cerebral cortex ventricular layer	Present		8_E16.5
TS24: cerebral cortex marginal layer	Trace		8_E16.5
TS24: cortical plate	Absent		8_E16.5

Structure	Level	Pattern	Image	Note
TS16: telencephalon	Present	Regionally restricted	9A	Expression is in neuroepithelial cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	cerebral cortex neuron	Present		10A	Expression is in neurons. Immunoreactivity along the lateral plasma membrane of neuroepithelial cells consistently showed a ventricular-high, pial-low gradient.
TS22: cerebral cortex ventricular layer	cerebral cortex neuron	Present		10A	Expression is in neurons. Immunoreactivity along the lateral plasma membrane of neuroepithelial cells consistently showed a ventricular-high, pial-low gradient.
TS22: cortical plate		Absent		10A

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	radial glial cell	Present		10C	Expression is in radial glial cell processes.
TS22: cerebral cortex marginal layer	radial glial cell	Present		10C	Expression is in radial glial cell processes.
TS22: cortical plate		Absent		10C

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present
TS20: spinal cord roof plate	Present

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex ventricular zone	Absent