Gene Expression Data
Assay Details
Assay
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Reference:
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J:227274
Bloomquist RF, et al., Coevolutionary patterning of teeth and taste buds. Proc Natl Acad Sci U S A. 2015 Nov 3;112(44):E5954-62
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7627306
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Gene symbol:
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Sfrp5
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Gene name:
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secreted frizzled-related sequence protein 5
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Probe:
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MGC clone 41101
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3D:
embryonic day 13.5
(more )
Specimen
3D:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: molar mesenchyme |
Present |
Regionally restricted |
3D
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Expression predominantly in buccal molar tooth mesenchyme.
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TS21: tooth bud |
Present |
Regionally restricted |
3D
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Expression predominantly in buccal molar tooth mesenchyme.
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TS21: molar epithelium |
Present |
Regionally restricted |
3D
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Some expression in epithelium at tip of bud.
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Specimen
3E:
embryonic day 13.5
(more )
Specimen
3E:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Specimen
3F:
embryonic day 13.5
(more )
Specimen
3F:
(close )
Genetic Background:
|
ICR
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Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
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Specimen
3D':
embryonic day 15.5
(more )
Specimen
3D':
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: molar mesenchyme |
Absent |
|
3D'
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Mesenchymal expression largely undetectable.
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TS23: cap stage tooth |
Present |
Regionally restricted |
3D'
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Highly restricted expression in epithelium of cervical loop with greater level in buccal aspect.
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TS23: cervical loop |
Present |
Regionally restricted |
3D'
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Highly restricted expression in epithelium of cervical loop with greater level in buccal aspect.
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Specimen
3E':
embryonic day 15.5
(more )
Specimen
3E':
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
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Specimen
3F':
embryonic day 15.5
(more )
Specimen
3F':
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: tongue epithelium |
Present |
Regionally restricted |
3F'
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Localized expression on dorsal and lateral epithelium surfaces.
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