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Gene Expression Data
Assay Details
Assay
Reference: J:242186 Hsieh CP, et al., Deficits in cerebellar granule cell development and social interactions in CD47 knockout mice. Dev Neurobiol. 2015 May;75(5):463-84
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7627151
Gene symbol: Cd47
Gene name: CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
Antibody: mIAP301 clone
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 2D: postnatal day 6 (more )
Structure Level Pattern Image Note
TS28: cerebellum molecular layer Strong Regionally restricted 2D Expression is in the inner layers.
TS28: cerebellum external granule cell layer Present Regionally restricted 2D Expression is in the inner layers.
TS28: cerebellum internal granule cell layer Present Regionally restricted 2D Expression is in the inner layers.
Specimen 2E: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: cerebellum internal granule cell layer Strong 2E
TS28: cerebellum molecular layer Strong 2E
TS28: cerebellum external granule cell layer Trace 2E
Specimen 2F: postnatal day 14 (more )
Structure Level Pattern Image Note
TS28: cerebellum internal granule cell layer Strong 2F
TS28: cerebellum molecular layer Strong 2F
TS28: cerebellum external granule cell layer Trace 2F
Specimen 3A: postnatal day 10 (more )
Note: Double labeled: red - Cd47; green - Mki67 (assay MGI:7627152).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum external granule cell layer cerebellar granule cell Present Non-Uniform 3A CD47 , 3A Merge Expression is strong in the inner layer and is weak in the outer layer of the external granule layer.
Specimen 3B: postnatal day 10 (more )
Note: Double labeled: red - Cd47; green - Cntn2 (assay MGI:7627154).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum external granule cell layer cerebellar granule cell Present Non-Uniform 3B Merge , 3B CD47 Expression is strong in the inner layer and is weak in the outer layer of the external granule layer.
Specimen 3C: postnatal day 10 (more )
Note: Double labeled: red - Cd47; green - Camk4 (assay MGI:7627156).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum internal granule cell layer cerebellar granule cell Present 3C CD47 , 3C Merge Expression is in cerebellar granule cells.
Specimen 3D: postnatal day 10 (more )
Note: Double labeled: red - Cd47; green - Calb1 (assay MGI:7627157).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Weak 3D Expression is in Purkinje cells.
Specimen 3E: postnatal day 10 (more )
Note: Double labeled: red - Cd47; green - Gfap (assay MGI:7627158).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum radial glial cell Trace 3E Expression is in radial fibers of radial glial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory