Antibody:

anti-Nkx6-1

Structure	Cell Type	Level	Pattern	Image	Note
TS20: meninges		Present	Regionally restricted	4A	Expression is in the ventral meninges of the spinal cord.
TS20: spinal cord		Present	Regionally restricted	4A	Expression is in the ventrolateral neuroepithelium.
TS20: spinal cord ventral horn	motor neuron	Present	Regionally restricted	4A	Expression is in motor neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: meninges		Present	Regionally restricted	4B	Expression in the ventral meninges of the spinal cord is unaffected in this mutant.
TS20: spinal cord		Weak	Regionally restricted	4B	Expression in the ventrolateral neuroepithelium has shifted ventrally in this mutant compared to wild-type.
TS20: spinal cord ventral horn	motor neuron	Weak	Regionally restricted	4B	Expression in motor neurons has shifted ventrally in this mutant compared to wild-type.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	4C	Expression is in the ventral spinal cord.
TS19: primitive meninges	Present	Regionally restricted	4C	Expression in the ventral meninges of the spinal cord.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Absent		4D	Expression is also absent in the ventral meninges of the spinal cord.

Structure	Level	Pattern	Image	Note
TS19: esophagus mesenchyme	Strong		4E
TS19: trachea mesenchyme	Strong	Non-Uniform	4E	Expression is weak or absent in the ventral tracheal mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: foregut mesenchyme	Strong		4F	Expression is in the dorsolateral mesenchyme of the mutant tracheoesophageal fistula. Expression in the ventral mesenchyme of the tracheoesophageal fistula is weak or absent.