Gene Expression Data
Assay Details
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Assay
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Reference:
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J:63174
Garces A, et al., GFRalpha 1 is required for development of distinct subpopulations of motoneuron. J Neurosci. 2000 Jul 1;20(13):4992-5000
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7626257
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Probe:
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Isl1 cDNA10
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 13.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1E:
embryonic day 13.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord lumbar region |
motor neuron |
Present |
Regionally restricted |
1E
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Expression in motoneurons in most motor columns.
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Specimen
4A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Gfra2tm1Msa/Gfra2tm1Msa
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4B:
embryonic day 15.5
(more )
Specimen
4B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Gfra1tm1Rosl/Gfra1tm1Rosl
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: spinal cord brachial region |
motor neuron |
Present |
Regionally restricted |
4C
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Authors report that expression was missing in a group of motoneurons that was present in wild type and Gfra2-/- mutants.
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Specimen
4G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Gfra2tm1Msa/Gfra2tm1Msa
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4H:
embryonic day 15.5
(more )
Specimen
4H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Gfra1tm1Rosl/Gfra1tm1Rosl
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: spinal cord lumbar region |
motor neuron |
Present |
Regionally restricted |
4I
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Authors report that expression was missing in a group of motoneurons that was present in wild type and Gfra2-/- mutants.
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Analysis Tools