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Gene Expression Data
Assay Details
Assay
Reference: J:40858 Dagnino L, et al., Expression patterns of the E2F family of transcription factors during murine epithelial development. Cell Growth Differ. 1997 May;8(5):553-63
Assay type: RNA in situ
MGI Accession ID: MGI:7624499
Gene symbol: E2f4
Gene name: E2F transcription factor 4
Probe: E2F-4 cDNA1
Probe preparation: Antisense labelled with P33 RNA
Visualized with: Autoradiography
Assay notes: Two probes were described MGI:7624462 and MGI:7624473. The one used in this assay was not specified.
Results
Specimen 3A/B: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: surface ectoderm Present Widespread 3A/B Expression was detected in all cells of the ectoderm.

Specimen 3C/D: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: epidermis Strong Regionally restricted 3C/D In 13.5-dpc embryos, the proliferating, undifferentiated epithelial cells showed strong expression. Expression in the underlying mesenchyme was lower.
TS21: hair follicle Strong Regionally restricted 3C/D Proliferating, undifferentiated epithelial cells around the dermal papilla showed strong expression.

Specimen 3E/F: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: epidermis Strong Regionally restricted 3E/F Mitotically active epithelial cells showed strong expression. Expression in the underlying mesenchyme was lower.

Specimen not shown: embryonic day 18.5-19.5 (more )
Structure Level Pattern Image Note
TS26: epidermis basal layer Strong
TS26: epidermis suprabasal layer Absent
TS26: hair follicle Strong Regionally restricted High expression levels were noted around the dermal papillae.
TS26: vibrissa follicle Strong Regionally restricted High expression levels were noted around the dermal papillae.

Specimen 6A/B: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: liver Present 6A/B
TS20: intestine Absent 6A/B

Specimen 6C/D: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: intestine epithelium Present 6C/D

Specimen not shown: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: intestine epithelium Strong

Specimen 6E/F: embryonic day 19.5 (more )
Structure Level Pattern Image Note
TS26: intestine epithelium Strong Regionally restricted 6E/F Expression was maintained in the intervillus epithelium.
TS26: intestinal villus Present Regionally restricted 6E/F Expression was detected in the lower portions of the developing villi.
TS26: intestine mesenchyme Absent 6E/F

Specimen Table 1 E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: surface ectoderm Present
TS19: intestine epithelium Absent

Specimen Table 1 E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: intestine epithelium Absent

Specimen Table 1 E13.5: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: intestine epithelium Present

Specimen Table 1 E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: intestine epithelium Present

Specimen Table 1 E15.5: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: epidermis basal layer Present
TS23: epidermis suprabasal layer Absent
TS23: hair follicle Present Regionally restricted Expression pattern in epithelial cells surrounding the dermal papilla.
TS23: intestine epithelium Present Regionally restricted Expression was detected in intervillus epithelium.
TS23: intestinal villus Present Regionally restricted Expression was detected at the base, but not the apex of the villi.

Specimen Table 1 E19.5: embryonic day 19.5 (more )
Structure Level Pattern Image Note
TS26: epidermis basal layer Present
TS26: epidermis suprabasal layer Absent
TS26: hair follicle Present Regionally restricted Expression pattern in epithelial cells surrounding the dermal papilla.
TS26: intestine epithelium Present Regionally restricted Expression was detected in intervillus epithelium.
TS26: intestinal villus Present Regionally restricted Expression was detected at the base, but not the apex of the villi.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory