Tg(RP23-157J4*)850Mhoa
Transgene Detail
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| Symbol: |
Tg(RP23-157J4*)850Mhoa |
| Name: |
transgene insertion 850, Michael Hoa |
| MGI ID: |
MGI:7623542 |
| Synonyms: |
Tg(Kcnj10-ZsGreen)skMHa |
| Transgene: |
Tg(RP23-157J4*)850Mhoa Location: unknown
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| Alliance: |
Tg(RP23-157J4*)850Mhoa page
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| Strain of Origin: |
B6SJLF1/J
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| Allele Type: |
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Targeted (Reporter) |
| Mutation: |
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Insertion
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Tg(RP23-157J4*)850Mhoa expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: A 186 kb bacterial artificial chromosome (BAC) clone RP23-157J4, spanning from Chr1:172,236,604-172,422,466 (GRCm38/mm10) was used to obtain mouse genomic DNA containing the entire mouse potassium inwardly-rectifying channel, subfamily J, member 10 (Kcnj10) promoter and regulatory sequences. This BAC was modified to replace the Kcnj10 coding sequence (genomic region corresponding to NM_001039484.1: exon 2 nucleotide 243-1393) with a ZsGreen expression cassette. The BAC contains numerous other genes in addition to Kcnj10, including Atp1a4 (partial), Igsf8, Atp1a2, Kcnj9, and Pigm which were not disabled or modified on the BAC Tg. The linearized BAC was microinjected into fertilized oocytes derived from breeding B6SJLF1/J mice together. Four transgenic founder mice were obtained by random integration. A founder line 850 with a similar ZsGreen expression pattern was selected. Line 850 has 2 copies of the transgene.
(J:347363)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:347363 Strepay D, et al., Transgenic Tg(Kcnj10-ZsGreen) fluorescent reporter mice allow visualization of intermediate cells in the stria vascularis. Sci Rep. 2024 Feb 6;14(1):3038 |
| All: |
1 reference(s) |
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Analysis Tools
