Gene Expression Data
Assay Details
Assay
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Reference:
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J:107740
McGrath J, et al., Two populations of node monocilia initiate left-right asymmetry in the mouse. Cell. 2003 Jul 11;114(1):61-73
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7621216
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Gene symbol:
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Dnah11
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Gene name:
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dynein, axonemal, heavy chain 11
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Reporter:
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GFP
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Direct Detection
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Results
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Specimen
2A:
(close )
Genetic Background:
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involves: 129S1/Sv * FVB/N
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Mutant Allele(s):
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Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
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Age:
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postnatal adult
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Sex:
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Female
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: oviduct ampulla |
ciliated epithelial cell |
Present |
Regionally restricted |
2A
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Expression was detected in ciliated epithelium.
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Specimen
2C:
(close )
Genetic Background:
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involves: 129S1/Sv * FVB/N
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Mutant Allele(s):
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Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
|
Age:
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postnatal adult
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Sex:
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Male
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2E:
(close )
Genetic Background:
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involves: 129S1/Sv * FVB/N
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Mutant Allele(s):
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Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
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Age:
|
embryonic day 7.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: node |
Present |
|
2E
|
|
Specimen
2F:
(close )
Genetic Background:
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involves: 129S1/Sv * FVB/N
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Mutant Allele(s):
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Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
|
Age:
|
postnatal adult
|
Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Dnah11; red - acetylated tubulin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: oviduct ampulla |
ciliated epithelial cell |
Present |
Regionally restricted |
2F
,
2H
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Expression was detected in the cilia, which were identified by anti-acetylated tubulin. No GFP fluorescence was observed in nonciliated cells, and no GFP fluorescence was seen in the cytoplasm of ciliated cells.
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Specimen
2L:
(close )
Genetic Background:
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involves: 129S1/Sv * FVB/N
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Mutant Allele(s):
|
Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Dnah11; red - acetylated tubulin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: choroid plexus |
ciliated epithelial cell |
Present |
Regionally restricted |
2N
,
2L
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Expression was detected in the cilia, which were identified by anti-acetylated tubulin. No GFP fluorescence was observed in nonciliated cells, and no GFP fluorescence was seen in the cytoplasm of ciliated cells.
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Specimen
2O:
(close )
Genetic Background:
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involves: 129S1/Sv * FVB/N
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Mutant Allele(s):
|
Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Dnah11; red - acetylated tubulin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: trachea |
ciliated epithelial cell |
Weak |
Regionally restricted |
2Q
,
2O
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Expression was detected in the cilia, which were identified by anti-acetylated tubulin. No GFP fluorescence was observed in nonciliated cells, and no GFP fluorescence was seen in the cytoplasm of ciliated cells.
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Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * FVB/N
|
Mutant Allele(s):
|
Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
Not Specified
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3B:
(close )
Genetic Background:
|
involves: 129S1/Sv * FVB/N
|
Mutant Allele(s):
|
Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
|
Age:
|
embryonic day 7.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: node |
Weak |
Regionally restricted |
3B
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Faint GFP fluorescence was visible as small dots in the ventral node cells.
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Specimen
3D:
(close )
Genetic Background:
|
involves: 129S1/Sv * FVB/N
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Mutant Allele(s):
|
Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
|
Age:
|
embryonic day 7.75
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Headfold stage.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS10: node |
ciliated cell |
Present |
|
3F
,
3D
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TS10: node |
|
Present |
Regionally restricted |
3D
,
3E
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GFP fluorescence was detected only in ciliated cells in the node. In a deeper section of cell bodies in the ventral node, no GFP fluorescence was identified.
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Specimen
3H:
(close )
Genetic Background:
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involves: 129S1/Sv * FVB/N
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Mutant Allele(s):
|
Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
|
Age:
|
embryonic day 7.75
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Dnah11; red - acetylated tubulin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS11: node |
ciliated cell |
Present |
Regionally restricted |
3H
,
3I
,
3K
,
3J
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GFP fluorescence was found in a centrally located subset of node monocilia, which were identified by anti-acetylated tubulin. No left-right asymmetry was noted in the distribution of GFP fluorescing cilia. A second subset of node monocilia does not show GFP fluorescence, predominantly found at the left, right and anterior margins of the node (31-33% of all cilia).
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Specimen
not shown E8.25:
(close )
Genetic Background:
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involves: 129S1/Sv * FVB/N
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Mutant Allele(s):
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Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
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Age:
|
embryonic day 8.25
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Sex:
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Not Specified
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Type:
|
Not Specified
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Fixation:
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4% Paraformaldehyde
|
Specimen
not shown E10.5:
(close )
Genetic Background:
|
involves: 129S1/Sv * FVB/N
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Mutant Allele(s):
|
Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
|
Age:
|
embryonic day 10.5
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Sex:
|
Not Specified
|
Type:
|
Not Specified
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Fixation:
|
4% Paraformaldehyde
|
Specimen
5B:
(close )
Genetic Background:
|
involves: 129S1/Sv * FVB/N
|
Mutant Allele(s):
|
Dnah11tm1.1Bknr/Dnah11tm1.1Bknr
|
Age:
|
embryonic day 7.75
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Dnah11 reporter; red - Pkd2 (assay MGI:7621236).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS11: node |
ciliated cell |
Present |
Regionally restricted |
5E
,
5C
,
5B
,
5H
,
5F
,
5I
|
GFP fluorescence was detected in central node monocilia. Central, motile monocilia were labeled for both Pkd2 and Dnah11-GFP reporter. Peripheral, immotile cilia were labeled exclusively for Pkd2.
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