Acan Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:170410 Sato T, et al., Chondroitin sulfate N-acetylgalactosaminyltransferase 1 is necessary for normal endochondral ossification and aggrecan metabolism. J Biol Chem. 2011 Feb 18;286(7):5803-12
Assay type:	Western blot
MGI Accession ID:	MGI:7620308
Gene symbol:	Acan
Gene name:	aggrecan

Antibody:	12/21/1-C-6
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

	Sample Information		Bands				Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (c)	200 kDa	100 kDa	Genetic Background	Mutant Allele(s)	Sex	Note
+/+	P newborn	TS27: articular cartilage TS27: tibia	Present (b)	Present	Present	Present	involves: C57BL/6J		Not Specified	Proteoglycans extracted from tibial cartilage after chondroitinase ABC treatment.
+/+	P newborn	TS27: articular cartilage TS27: tibia	Present (b)	Present	Present	Present	involves: C57BL/6J		Not Specified	Proteoglycans extracted from tibial cartilage after chondroitinase ABC treatment.
+/+	P newborn	TS27: articular cartilage TS27: tibia	Present (b)	Present	Present	Present	involves: C57BL/6J		Not Specified	Proteoglycans extracted from tibial cartilage after chondroitinase ABC treatment.
-/-	P newborn	TS27: articular cartilage TS27: tibia	Weak (d)	Weak (d)	Present (e)	Present	involves: C57BL/6J	Csgalnact1^tm1.1Nari/Csgalnact1^tm1.1Nari	Not Specified	Proteoglycans extracted from tibial cartilage after chondroitinase ABC treatment.
-/-	P newborn	TS27: articular cartilage TS27: tibia	Weak (d)	Weak (d)	Present (e)	Present	involves: C57BL/6J	Csgalnact1^tm1.1Nari/Csgalnact1^tm1.1Nari	Not Specified	Proteoglycans extracted from tibial cartilage after chondroitinase ABC treatment.
-/-	P newborn	TS27: articular cartilage TS27: tibia	Weak (d)	Weak (d)	Present (e)	Present	involves: C57BL/6J	Csgalnact1^tm1.1Nari/Csgalnact1^tm1.1Nari	Not Specified	Proteoglycans extracted from tibial cartilage after chondroitinase ABC treatment.

Notes:
(a) Higher band >300 kDa, full-length.
(b) major band
(c) Lower band >300 kDa
(d) Substantially diminished by comparison with the wild-type.
(e) Major band, with slightly greater signal intensity than that of the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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