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Gene Expression Data
Assay Details
Assay
Reference: J:330334 Zhang H, et al., The RNA ligase RNA terminal phosphate cyclase B regulates mRNA alternative splicing and is required for mouse oocyte development and maintenance. Development. 2022 Oct 1;149(19):dev200497
Assay type: RT-PCR
MGI Accession ID: MGI:7620118
Gene symbol: Rad50
Gene name: RAD50 double strand break repair protein
Probe: Rad50-intron22-pF, Rad50-intron22-IR-pR
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
IR WT P adult TS28: primary oocyte Trace Not Specified; total RNA involves: C57BL/6J Female
IR Rtcb oo-/- P adult TS28: primary oocyte Present (a) Not Specified; total RNA involves: C57BL/6J Rtcbtm1Hyfn/Rtcbtm1Hyfn, Tg(Gdf9-icre)5092Coo/0Female Conditional mutant.
Total WT Other lane(s) in blot use different probe(s)
Total Rtcb oo-/- Other lane(s) in blot use different probe(s)
Notes:
(a) Intron retension occurred in these transcripts.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory