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Gene Expression Data
Assay Details
Assay
Reference: J:330334 Zhang H, et al., The RNA ligase RNA terminal phosphate cyclase B regulates mRNA alternative splicing and is required for mouse oocyte development and maintenance. Development. 2022 Oct 1;149(19):dev200497
Assay type: RT-PCR
MGI Accession ID: MGI:7620103
Gene symbol: Rtcb
Gene name: RNA 2',3'-cyclic phosphate and 5'-OH ligase
Probe: Rtcb-pF, Rtcb-pR
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh.
Results
Image: 1B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
GV P adult TS28: primary oocyte Present (a) Not Specified; total RNA involves: C57BL/6J Female
MII P adult TS28: secondary oocyte Present (a) Not Specified; total RNA involves: C57BL/6J Female
1-cell E0.5 (b) TS1: 1-cell stage embryo Present Not Specified; total RNA involves: C57BL/6J Not Specified
2-cell E1.0 (b) TS2: 2-cell stage embryo Weak Not Specified; total RNA involves: C57BL/6J Not Specified
4-cell E2.0 (b) TS3: 4-cell stage embryo Strong (c) Not Specified; total RNA involves: C57BL/6J Not Specified
8-cell E2.5 (b) TS3: 8-cell stage embryo Very strong (c) Not Specified; total RNA involves: C57BL/6J Not Specified
Morula E3.0 (b) TS4: compacted morula Very strong (c) Not Specified; total RNA involves: C57BL/6J Not Specified
Blasocyst E3.5 (b) TS5: blastocyst Present Not Specified; total RNA involves: C57BL/6J Not Specified
Notes:
(a) Expression remained relatively stable in oocytes.
(b) Age assigned by curator based on morphological criteria supplied by authors.
(c) Expression increased drastically after the two-cell stage.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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