Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS21: otic ganglion	Present		4A
TS21: sphenopalatine ganglion	Present		4A
TS21: superior cervical ganglion	Present		4A
TS21: facial ganglion	Present		4A	geniculate ganglion
TS21: facial nerve	Present	Regionally restricted	4A	Expressed in the intermediate nerve, facial motor root, and proximal part, but was absent in the distal portion of the facial nerve beyond the branch point with the chorda tympani.
TS21: cranial nerve	Present	Regionally restricted	4A	Expressed in the greater superficial petrosal nerve.

Structure	Level	Pattern	Image	Note
TS21: facial ganglion	Present		4B	geniculate ganglion
TS21: facial nerve	Present		4B
TS21: superior cervical ganglion	Present		4B
TS21: cranial nerve	Present	Regionally restricted	4B	The greater superficial petrosal nerve was absent, which is replaced by a stump that protruded from the geniculate ganglion.

Structure	Level	Pattern	Image	Note
TS24: otic ganglion	Present		5A	More intensely stained compared with that of E13.5.
TS24: sphenopalatine ganglion	Present		5A	More intensely stained compared with that of E13.5.
TS24: superior cervical ganglion	Present		5A	More intensely stained compared with that of E13.5.
TS24: cranial nerve	Present	Regionally restricted	5A	Expressed in the greater superficial petrosal nerve and vidian nerve.
TS24: facial ganglion	Absent		5A	Cells in the geniculate ganglion were no longer detectably labeled, and the ganglion was visualized owing to the visceral motor fibers coursing through it.

Structure	Level	Pattern	Image	Note
TS24: sphenopalatine ganglion	Trace	Regionally restricted	5B	In homozygous mutant embryos, the sphenopalatine ganglion was absent or reduced to a few clusters of cells.
TS24: cranial nerve	Present	Regionally restricted	5B	The greater superficial petrosal nerve was absent, which is replaced by a stump.

Structure	Level	Pattern	Image	Note
TS24: otic ganglion	Present		5C
TS24: facial ganglion	Absent		5C	Cells in the geniculate ganglion were no longer detectably labeled, and the ganglion was visualized owing to the visceral motor fibers coursing through it.

Structure	Level	Pattern	Image	Note
TS24: otic ganglion	Present		5D	Additional nerve was seen projecting from the otic ganglion in the homozygous mutant.

Structure	Level	Pattern	Image	Note
TS24: otic ganglion	Present		5E	Additional nerve was seen projecting from the otic ganglion in the homozygous mutant.

Structure	Level	Pattern	Image	Note
TS24: cranial nerve	Present	Regionally restricted	5F	Sympathetic axons had fasciculated with the greater superficial petrosal nerve to form the vidian nerve.

Structure	Level	Pattern	Image	Note
TS24: cranial nerve	Present	Regionally restricted	5G	Sympathetic axons had failed to extend rostrally beyond the point they reached at E13.5. The ectopic nerve emanating from the otic ganglion was seen, which did not fasciculate with the sympathetic fibers.

Structure	Level	Pattern	Image	Note
TS21: facial ganglion	Present		5H
TS21: otic ganglion	Present		5H
TS21: sphenopalatine ganglion	Present		5H
TS21: sublingual ganglion	Present		5H
TS21: submandibular ganglion	Present		5H
TS21: cranial nerve	Present		5H	Rostrally directed sympathetic axons were seen, joined by the greater superficial petrosal nerve, forming the vidian nerve.

Structure	Level	Pattern	Image	Note
TS21: cranial nerve	Present		5I	The rostrally directed sympathetic axons were also present in homozygous mutant.
TS21: facial ganglion	Present		5I

Structure	Level	Pattern	Image	Note
TS22: otic ganglion	Present		6A
TS22: sphenopalatine ganglion	Present		6A
TS22: cranial nerve	Present	Regionally restricted	6A	The greater superficial petrosal nerve and vidian nerve were clearly seen.

Structure	Level	Pattern	Image	Note
TS22: otic ganglion	Present		6B	Much weaker than in control embryos.
TS22: sphenopalatine ganglion	Present		6B	Much weaker than in control embryos.
TS22: cranial nerve	Present		6B	Persistence of the greater superficial petrosal nerve and vidian nerves were seen in null mutant embryos.