Gene Expression Data
Assay Details
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Assay
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Reference:
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J:60735
Bumsted KM, et al., Dorsal retinal pigment epithelium differentiates as neural retina in the microphthalmia (mi/mi) mouse. Invest Ophthalmol Vis Sci. 2000 Mar;41(3):903-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7619958
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Gene symbol:
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Nefl
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Gene name:
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neurofilament, light polypeptide
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Antibody:
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8A1
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Detection system:
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Secondary antibody coupled to Texas Red
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Results
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Specimen
1G:
embryonic day 17.5
(more  )
Specimen
1G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1H:
(close )
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Genetic Background:
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B6C3Fe-a/a
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Mutant Allele(s):
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MitfMi/MitfMi
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: retina ganglion cell layer |
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Present |
Regionally restricted |
1H
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The same cell types were labeled in the mutant neural retina as seen in the wild type.
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| TS25: neural retina |
retina horizontal cell |
Present |
Regionally restricted |
1H
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The same cell types were labeled in the mutant neural retina as seen in the wild type. Expression was detected in migrating horizontal cells.
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Specimen
1I:
(close )
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Genetic Background:
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B6C3Fe-a/a
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Mutant Allele(s):
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MitfMi/MitfMi
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: pigmented retinal epithelium |
Present |
Regionally restricted |
1I
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At E17, masses of neurites in the most scleral part of the dorsal thickened retinal pigmented epithelium were immunolabeled, which is normally never expressed in the retinal pigmented epithelium layer.
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Analysis Tools
