Gene Expression Data
Assay Details
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Assay
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Reference:
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J:60735
Bumsted KM, et al., Dorsal retinal pigment epithelium differentiates as neural retina in the microphthalmia (mi/mi) mouse. Invest Ophthalmol Vis Sci. 2000 Mar;41(3):903-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7619954
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Antibody:
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anti-Otx2
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Detection system:
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Secondary antibody coupled to Texas Red
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Results
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Specimen
1E:
embryonic day 13.5-14.5
(more  )
Specimen
1E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5-14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neural retina |
Present |
Regionally restricted |
1E
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Expression was detected in cell nuclei in the central neural retina.
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| TS21: pigmented retinal epithelium |
Present |
Regionally restricted |
1E
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Expression was detected in a row of retinal pigmented epithelium nuclei.
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Specimen
1F:
(close )
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Genetic Background:
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B6C3Fe-a/a
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Mutant Allele(s):
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MitfMi/MitfMi
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Age:
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embryonic day 13.5-14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neural retina |
Present |
Scattered |
1F
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Expression was detected in scattered cell nuclei in the central neural retina.
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| TS21: pigmented retinal epithelium |
Present |
Regionally restricted |
1F
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Expression was detected in the retinal pigmented epithelium regions that remained a monolayer. The dorsal expanded region contained little staining.
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Specimen
3G:
postnatal day 17
(more )
Specimen
3G:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 17
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3H:
(close )
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Genetic Background:
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B6C3Fe-a/a
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Mutant Allele(s):
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MitfMi/MitfMi
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Age:
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postnatal day 17
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
retinal bipolar neuron |
Present |
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3H
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Fewer cells were indicated in the mutant inner nuclear layer.
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Specimen
3I:
(close )
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Genetic Background:
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B6C3Fe-a/a
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Mutant Allele(s):
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MitfMi/MitfMi
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Age:
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postnatal day 17
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
retinal bipolar neuron |
Present |
Regionally restricted |
3I
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In the dorsal expanded region of the mutant, immunolabeled bipolar cells were detected in an ectopic inner nuclear layer.
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Analysis Tools
