Antibody:

anti-Atrx (H-300, sc-15408)

Structure	Level	Pattern	Image	Note
TS11: trophoblast giant cell	Very strong	Regionally restricted	5E , 5B left	Very high levels of expression in trophoblast giant cells surrounding the Reichert's membrane.
TS11: embryo	Strong		5C left , 5B left	Highest expression.
TS11: neural ectoderm	Strong		5C left , 5B left	Highest expression.
TS11: chorion	Strong		5D left , 5B left	Highest expression.
TS11: ectoplacental cone	Present		5D left , 5B left	Detectable but lower levels of expression.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		5B left

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		5C right , 5B right	Only background staining was seen.
TS11: neural ectoderm	Absent		5C right , 5B right	Only background staining was seen.
TS11: chorion	Absent		5D right , 5B right	Only background staining was seen.
TS11: ectoplacental cone	Absent		5D right , 5B right	Only background staining was seen.
TS11: trophoblast giant cell	Absent		5B right	Only background staining was seen.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		5B right	Similar as in the wild-type.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		8A left , 8B left	Expression was detected in every cell.
TS11: chorion ectoderm	Present		8A left , 8C left
TS11: chorion mesoderm	Present		8A left , 8C left

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Clusters	8A right , 8B right	Expressed as a mosaic of small clusters of positive cells (in which the Atrxnull allele on Xm had been inactivated) and negative cells (in which the wild-type allele on Xp had been inactivated), indicating normal random X-inactivation within the epiblast.
TS11: chorion ectoderm	Present		8A right , 8C right	Expression could be detected in almost all cells, indicating the wild-type allele (inherited on the Xp chromosome) escaped the normal imprinted X-inactivation in the extraembryonic compartment.
TS11: chorion mesoderm	Present		8A right , 8C right	Expression could be detected in almost all cells, indicating the wild-type allele (inherited on the Xp chromosome) escaped the normal imprinted X-inactivation in the extraembryonic compartment.

Structure	Level	Pattern	Image	Note
TS12: neural fold	Present	Non-Uniform	S4A	Clearly comprised of a mosaic of positive and negative cells due to random X-inactivation.

Structure	Level	Pattern	Image	Note
TS12: primitive endoderm	Trace		S4B	Poorly expressed in the visceral endoderm.
TS12: chorion ectoderm	Strong		S4B	Strong expression in the chorionic ectoderm, indicating that the paternally-derived allele had escaped inactivation in this tissue.