Gene Expression Data
Assay Details
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Assay
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Reference:
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J:277128
Shiromoto Y, et al., GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice. Biol Reprod. 2019 Jul 1;101(1):248-256
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7619073
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Gene symbol:
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Piwil4
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Gene name:
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piwi-like RNA-mediated gene silencing 4
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Results
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Specimen
3C +/delta:
embryonic day 16.5; Gpat2tm1.1Nkn/Gpat2 +
(more  )
Specimen
3C +/delta:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2+
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: germ cell of testis |
Present |
Regionally restricted |
3C Piwil4 +/delta
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Secondary piRNAs are incorporated into PIWIL4, and the resultant PIWIL4-piRNA complexes are transferred into the nucleus.
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Specimen
3C delta/delta:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2tm1.1Nkn
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: germ cell of testis |
Present |
Regionally restricted |
3C Piwil4 delta/delta
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PIWIL4 proteins that do not bind to piRNAs cannot enter the nucleus. PIWIL4
was not detectable in the nucleus of Gpat2delta/delta prospermatogonia.
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Analysis Tools