Gene Expression Data
Assay Details
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Assay
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Reference:
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J:277128
Shiromoto Y, et al., GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice. Biol Reprod. 2019 Jul 1;101(1):248-256
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7619070
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Gene symbol:
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Ddx4
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Gene name:
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DEAD box helicase 4
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Results
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Specimen
2B +/delta:
embryonic day 16.5; Gpat2tm1.1Nkn/Gpat2 +
(more  )
Specimen
2B +/delta:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2+
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: germ cell of testis |
Present |
Regionally restricted |
2B Ddx4 +/delta
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Expression was detected in a granular pattern in prospermatogonia.
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Specimen
2B delta/delta:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2tm1.1Nkn
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: germ cell of testis |
Present |
Regionally restricted |
2B Ddx4 delta/delta
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The immunopositive granules were not significantly changed in the mutant.
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Specimen
4A +/+:
postnatal day 7
(more )
Specimen
4A +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4A +/delta:
postnatal day 7; Gpat2tm1.1Nkn/Gpat2 +
(more )
Specimen
4A +/delta:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2+
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4A delta/delta left:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2tm1.1Nkn
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatogonium |
Present |
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4A delta/delta left
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A significant loss of germ cells was observed in the mutant.
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Specimen
4A delta/delta right:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2tm1.1Nkn
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatogonium |
Present |
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4A delta/delta right
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A significant loss of germ cells was observed in the mutant.
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Specimen
4C P3 +/delta left:
postnatal day 3; Gpat2tm1.1Nkn/Gpat2 +
(more )
Note: Double labeled: red - Ddx4; green - TUNEL.
Specimen
4C P3 +/delta left:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2+
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Age:
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postnatal day 3
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ddx4; green - TUNEL.
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Specimen
4C P3 +/delta right:
postnatal day 3; Gpat2tm1.1Nkn/Gpat2 +
(more )
Note: Double labeled: red - Ddx4; green - TUNEL.
Specimen
4C P3 +/delta right:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2+
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Age:
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postnatal day 3
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ddx4; green - TUNEL.
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Specimen
4C P3 delta/delta left:
postnatal day 3; Gpat2tm1.1Nkn/ Gpat2tm1.1Nkn
(more )
Note: Double labeled: red - Ddx4; green - TUNEL.
Specimen
4C P3 delta/delta left:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2tm1.1Nkn
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Age:
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postnatal day 3
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ddx4; green - TUNEL.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: spermatogonium |
Present |
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4C P3 delta/delta left
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A significant loss of germ cells was observed in the mutant.
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Specimen
4C P3 delta/delta right:
postnatal day 3; Gpat2tm1.1Nkn/ Gpat2tm1.1Nkn
(more )
Note: Double labeled: red - Ddx4; green - TUNEL.
Specimen
4C P3 delta/delta right:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2tm1.1Nkn
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Age:
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postnatal day 3
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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Double labeled: red - Ddx4; green - TUNEL.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: spermatogonium |
Present |
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4C P3 delta/delta right
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A significant loss of germ cells was observed in the mutant.
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Specimen
4C P7 +/delta:
postnatal day 7; Gpat2tm1.1Nkn/Gpat2 +
(more )
Note: Double labeled: red - Ddx4; green - TUNEL.
Specimen
4C P7 +/delta:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2+
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ddx4; green - TUNEL.
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Specimen
4C P7 delta/delta left:
postnatal day 7; Gpat2tm1.1Nkn/ Gpat2tm1.1Nkn
(more )
Note: Double labeled: red - Ddx4; green - TUNEL.
Specimen
4C P7 delta/delta left:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2tm1.1Nkn
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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Double labeled: red - Ddx4; green - TUNEL.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatogonium |
Present |
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4C P7 delta/delta left
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A significant loss of germ cells was observed in the mutant.
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Specimen
4C P7 delta/delta right:
postnatal day 7; Gpat2tm1.1Nkn/ Gpat2tm1.1Nkn
(more )
Note: Double labeled: red - Ddx4; green - TUNEL.
Specimen
4C P7 delta/delta right:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2tm1.1Nkn
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
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Double labeled: red - Ddx4; green - TUNEL.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatogonium |
Present |
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4C P7 delta/delta right
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A significant loss of germ cells was observed in the mutant.
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Specimen
S5A P2 +/delta:
postnatal day 2; Gpat2tm1.1Nkn/Gpat2 +
(more )
Specimen
S5A P2 +/delta:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2+
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Age:
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postnatal day 2
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S5A P2 delta/delta:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2tm1.1Nkn
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Age:
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postnatal day 2
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of testis |
Present |
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S5A P2 Gpat2delta/delta
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The number of germ cells was slightly reduced in the Gpat2 mutant mice.
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Specimen
S5A P5 +/delta upper:
postnatal day 5; Gpat2tm1.1Nkn/Gpat2 +
(more )
Specimen
S5A P5 +/delta upper:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2+
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Age:
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postnatal day 5
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Sex:
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Male
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S5A P5 +/delta lower:
postnatal day 5; Gpat2tm1.1Nkn/Gpat2 +
(more )
Specimen
S5A P5 +/delta lower:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2+
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Age:
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postnatal day 5
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Sex:
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Male
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S5A P5 delta/delta upper:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2tm1.1Nkn
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Age:
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postnatal day 5
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Sex:
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Male
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: germ cell of testis |
Present |
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S5A P5 upper Gpat2delta/delta
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The reduction in the number of germ cells was much more severe in Gpat2-deficient mice compared with Piwil2-/- mice.
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Specimen
S5A P5 delta/delta lower:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6NSlc
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Mutant Allele(s):
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Gpat2tm1.1Nkn/Gpat2tm1.1Nkn
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Age:
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postnatal day 5
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Sex:
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Male
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Type:
|
section
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|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: germ cell of testis |
Present |
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S5A P5 lower Gpat2delta/delta
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The reduction in the number of germ cells was much more severe in Gpat2-deficient mice compared with Piwil2-/- mice.
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Specimen
S5A P2 +/-:
postnatal day 2; Piwil2tm1Nkn/Piwil2 +
(more )
Specimen
S5A P2 +/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Piwil2tm1Nkn/Piwil2+
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Age:
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postnatal day 2
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S5A P2 -/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Piwil2tm1Nkn/Piwil2tm1Nkn
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Age:
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postnatal day 2
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Sex:
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Male
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of testis |
Present |
|
S5A P2 Piwil2-/-
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The number of germ cells was slightly reduced in the Piwil2 mutant mice.
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Specimen
S5A P5 +/- upper:
postnatal day 5; Piwil2tm1Nkn/Piwil2 +
(more )
Specimen
S5A P5 +/- upper:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
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Mutant Allele(s):
|
Piwil2tm1Nkn/Piwil2+
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Age:
|
postnatal day 5
|
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Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S5A P5 +/- lower:
postnatal day 5; Piwil2tm1Nkn/Piwil2 +
(more )
Specimen
S5A P5 +/- lower:
(close )
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Genetic Background:
|
involves: 129S2/SvPas
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Mutant Allele(s):
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Piwil2tm1Nkn/Piwil2+
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Age:
|
postnatal day 5
|
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Sex:
|
Male
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Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S5A P5 -/- upper:
(close )
|
Genetic Background:
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involves: 129S2/SvPas
|
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Mutant Allele(s):
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Piwil2tm1Nkn/Piwil2tm1Nkn
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|
Age:
|
postnatal day 5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: germ cell of testis |
Present |
|
S5A P5 upper Piwil2-/-
|
The reduction in the number of germ cells was much more severe in Gpat2-deficient mice compared with Piwil2-/- mice.
|
Specimen
S5A P5 -/- lower:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
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Mutant Allele(s):
|
Piwil2tm1Nkn/Piwil2tm1Nkn
|
|
Age:
|
postnatal day 5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: germ cell of testis |
Present |
|
S5A P5 lower Piwil2-/-
|
The reduction in the number of germ cells was much more severe in Gpat2-deficient mice compared with Piwil2-/- mice.
|
Specimen
S5B WT:
postnatal day 5
(more )
Specimen
S5B WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S5B +/delta:
postnatal day 5; Gpat2tm1.1Nkn/Gpat2 +
(more )
Specimen
S5B +/delta:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6NSlc * DBA/2
|
|
Mutant Allele(s):
|
Gpat2tm1.1Nkn/Gpat2+
|
|
Age:
|
postnatal day 5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S5B delta/delta:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6NSlc * DBA/2
|
|
Mutant Allele(s):
|
Gpat2tm1.1Nkn/Gpat2tm1.1Nkn
|
|
Age:
|
postnatal day 5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: germ cell of testis |
Present |
|
S5B delta/delta
|
Reduction in the number of germ cells also occurred in the DBA/2 background.
|
|
Analysis Tools