Antibody:	Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Present		2Ba
TS22: artery smooth muscle	Present	Regionally restricted	2Ba	Expression was detected in the smooth muscle layers of the pharyngeal arch arteries.

Structure	Level	Pattern	Image	Note
TS22: artery smooth muscle	Present		2Bb	Phenotypic defects were noted in the mutant.
TS22: cardiac muscle tissue	Present		2Bb	Phenotypic defects were noted in the mutant.

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Present		2Ca
TS23: artery smooth muscle	Present	Regionally restricted	2Ca	Expression was detected in the smooth muscle layers of the pharyngeal arch arteries.

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Present		2Cb
TS23: artery smooth muscle	Present	Regionally restricted	2Cb	Expression was detected in the smooth muscle layers of the pharyngeal arch arteries.

Structure	Level	Pattern	Image	Note
TS23: artery smooth muscle	Present		2Cc	Phenotypic defects were noted in the mutant.
TS23: cardiac muscle tissue	Present		2Cc	Phenotypic defects were noted in the mutant.

Structure	Level	Pattern	Image	Note
TS23: artery smooth muscle	Present		2Cd	Phenotypic defects were noted in the mutant.
TS23: cardiac muscle tissue	Present		2Cd	Phenotypic defects were noted in the mutant.