Antibody:	anti-PIP5Kgamma
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Immuno-electron microscopy, counterstained with uranyl acetate.

Structure	Cell Type	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	3a	Curved line pattern of expression in polygonal/oblong cardiomyocytes of early differentiation. Intense expression along plasma membranes at focal sites and in subjacent cytoplasm.

Structure	Cell Type	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	3b	Expression in cytoplasm of cardiomyocytes. Expression in short myofibrils located away from expression in cytoplasm.

Structure	Cell Type	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	3c	Expression in cytoplasm of cardiomyocytes. Expression in myofibrils embedded in areas of expression in cytoplasm, as well as in myofibrils located away from cytoplasmic expression.

Structure	Cell Type	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	4a	Expression in elongated cardiomyocytes of advanced differentation. Intense expression in straight-lined pattern. Expression in individual myofilaments of long myofibril bundles in spot form. Expression in some immature intercalated discs and Z-bands. Expression in focal sites on plasma membranes.

Structure	Cell Type	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	4b	Expression in individual myofilaments of long myofibril bundles in spot form. Expression in some immature intercalated discs and Z-bands.

Structure	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	Present	Regionally restricted	4c	Expression in some immature intercalated discs and Z-bands.

Structure	Cell Type	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	4d	Expression in Z-bands without expression.

Structure	Cell Type	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	4e	Portions of long myofibril bundles without expression.

Structure	Cell Type	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	4f	Expression in deposits on thicker myofilaments.

Structure	Cell Type	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	4g	Thinner myofilaments without expression.

Structure	Cell Type	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	4h	No expression on thinner myofilaments.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	6a	Intense expression in deposits in associated with most Z-bands and some intercalated discs.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	6b	Intense expression in deposits in associated with most Z-bands and some intercalated discs.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	6c	Intense expression in deposits in associated with some intercalated discs.