Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:346067
Ratchatasunthorn A, et al., Temporal involvement of phosphatidylinositol 4-phosphate 5-kinase gamma in differentiation of Z-bands and myofilament bundles as well as intercalated discs in mouse heart at mid-gestation. J Anat. 2024 Jan 26;
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7616414
|
|
Gene symbol:
|
Pip5k1c
|
|
Gene name:
|
phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma
|
|
Antibody:
|
anti-PIP5Kgamma
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
|
Results
|
Specimen
1a:
embryonic day 10.0
(more  )
Specimen
1a:
(close  )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: heart atrium |
Very strong |
|
1a
|
Selectively intense expression in heart in contrast to almost all surrounding regions, at levels distinctly different from them.
|
| TS16: primitive ventricle |
Very strong |
|
1a
|
Selectively intense expression in heart in contrast to almost all surrounding regions, at levels distinctly different from them.
|
Specimen
1b:
embryonic day 14.0
(more )
Specimen
1b:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: heart atrium |
Strong |
|
1b
|
Expression in heart at E14 at levels higher than that of surrounding organs and tissues, but not at levels distinctly different from them.
|
| TS22: heart ventricle |
Strong |
|
1b
|
Expression in heart at E14 at levels higher than that of surrounding organs and tissues, but not at levels distinctly different from them.
|
Specimen
1c:
embryonic day 18.0
(more )
Specimen
1c:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: heart atrium |
Present |
|
1c
|
Marked decrease in intensity of expression, without distinct difference from surrounding organs and tissues.
|
| TS26: heart ventricle |
Present |
|
1c
|
Marked decrease in intensity of expression, without distinct difference from surrounding organs and tissues.
|
Specimen
1d:
embryonic day 10.0
(more )
Specimen
1d:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: liver |
Present |
|
1d
|
|
Specimen
1e:
embryonic day 14.0
(more )
Specimen
1e:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: liver |
Present |
|
1e
|
|
Specimen
1f:
embryonic day 18.0
(more )
Specimen
1f:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: liver |
Present |
|
1f
|
|
Specimen
1g:
embryonic day 10.0
(more )
Specimen
1g:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
1h:
embryonic day 14.0
(more )
Specimen
1h:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
1i:
embryonic day 18.0
(more )
Specimen
1i:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2:
embryonic day 10.0
(more )
Specimen
2:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: heart |
Strong |
Regionally restricted |
2
|
Intense expression in cardiac walls and trabeculae.
|
Specimen
5a:
embryonic day 14.0
(more )
Specimen
5a:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: heart |
Strong |
Regionally restricted |
5a
|
Authors report frequent occurrence of straight patterns of intense expression with rare curved patterns of intense expression in walls and trabeculae.
|
Specimen
5b:
embryonic day 14.0
(more )
Specimen
5b:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: heart trabecula |
Present |
Regionally restricted |
5b
|
Expression in distinct striations at right angles to trajectory of some straight fibers.
|
|
Analysis Tools