Probe:	Lynx2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		2A
TS17: 2nd branchial arch	Present		2A
TS17: 3rd branchial arch	Present		2A
TS17: neural tube	Present	Regionally restricted	2A	Expression is in the cervical neural tube.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial arch	Present		2D
TS17: facial ganglion	Present		2D
TS17: glossopharyngeal ganglion	Present		2D
TS17: vagus ganglion	Present		2D
TS17: acoustic ganglion	Absent
TS17: trigeminal ganglion	Absent

Structure	Cell Type	Level	Pattern	Image	Note
TS17: neural tube	spinal cord motor neuron	Present		2G	Expression is in motor neurons.
TS17: dorsal root ganglion		Absent		2G

Structure	Cell Type	Level	Pattern	Image	Note
TS19: hindbrain		Present	Regionally restricted	2J	Expression is in the forming trigeminal (V) and ambiguus (IX/X) nuclei and in the lateral region.
TS19: neural tube	spinal cord motor neuron	Present	Regionally restricted	2J	Expression is in the ventro-lateral neural tube at the brachial level in motor neurons and in a few caudal cells.

Structure	Level	Pattern	Image	Note
TS20: paravertebral ganglion	Present	Regionally restricted	3A	Expression is in the stellate ganglion.
TS20: cervical ganglion	Present	Regionally restricted	3A	Expression is in the superior cervical ganglion.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: vagus ganglion	neuron	Present	Single cells	3C	Expression is in some neurons of the ganglion of the vagal (X) nerve.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: glossopharyngeal ganglion	sensory neuron	Present	Regionally restricted	3E	Expression is in a subset of sensory neurons in cervical sensory ganglia.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: sympathetic ganglion	neuron	Present	Regionally restricted	3F	Expression is in a subset of neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: dorsal root ganglion	sensory neuron	Present	Single cells	3G	Expression is in a few neurons, more strongly at rostral levels.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: glossopharyngeal ganglion	motor neuron	Strong		4B
TS20: trigeminal ganglion	motor neuron	Strong		4B
TS20: vagus ganglion	motor neuron	Strong		4B
TS20: spinal cord	interneuron	Present	Regionally restricted	4B	Expression is in a subpopulation of dorsal interneurons all along the rostro-caudal axis of the spinal cord.
TS20: spinal cord thoracic region	spinal cord motor neuron	Present	Regionally restricted	4B	Expression is in motor neurons of the most caudal part of the thoracic spinal cord.
TS20: spinal cord brachial region	spinal cord motor neuron	Present		4B	Expression is in motor neurons.
TS20: spinal cord lumbar region	spinal cord motor neuron	Present		4B	Expression is in motor neurons.
TS20: brainstem nucleus		Weak	Regionally restricted	4B	Expression is in the oculomotor (III), the trochlear (IV), the facial (VII) and the hypoglossal (XII) nuclei.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: spinal cord brachial region	spinal cord motor neuron	Present		4F	Expression is in brachial motor neurons.
TS20: spinal cord	interneuron	Present	Regionally restricted	4F	Expression is in both ventral and dorsal interneurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: spinal cord	interneuron	Present	Regionally restricted	4G	Expression is lateral in dI5 interneurons.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		5A
TS28: hippocampus CA1	Present		5A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer		Present	Single cells	5D
TS28: cerebellum Purkinje cell layer		Present	Single cells	5D	Expression is in distinct cells and is absent in the Purkinje cells.
TS28: cerebellum Purkinje cell layer	Purkinje cell	Absent		5D	Expression is in distinct cells and is absent in the Purkinje cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer IV	neuron	Present	Single cells	5G	Expression is in some projection neurons.
TS28: cortical layer V	neuron	Present	Single cells	5G	Expression is in some projection neurons.

Structure	Level	Pattern	Image	Note
TS20: hand	Present	Regionally restricted	6J	Expression is at the base of the forming digits.

Structure	Level	Pattern	Image	Note
TS20: hand	Present		6L	Expression in this mutant is the same as in wild-type.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present

Structure	Level	Pattern	Image	Note
TS20: facial ganglion	Present
TS20: sympathetic nerve trunk	Present	Single cells		Expression is in specific cells in the sympathetic trunk and pelvic ganglia.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: somatic motor system	motor neuron	Present			Expression is in somatic motor neurons.
TS28: spinal cord dorsal horn	interneuron	Present			Expression is in some interneurons of the dorsal horn.