Probe:	Mm-Irx1 (513861)
Probe preparation:	Antisense DNA
Visualized with:	Fluorescence

Structure	Level	Pattern	Image	Note
TS20: tooth bud	Present	Regionally restricted	4B In	Expression was detected in the non-initiation knot basal layer of the incisor.

Structure	Level	Pattern	Image	Note
TS20: tooth bud	Present	Regionally restricted	4B Mo	Expression was detected in the non-initiation knot basal layer of the molar.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	7A' , 7A	Expression was detected in the majority of ectodermal cells and border the Shh-positive endoderm.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	7B , 7B'	Irx1+ cells next to the developing heart now also express Cxcl14.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	7C , 7C'	Expression expanded noticeably between E10.0 and E10.5. Posteriorly, expression continued to adjoin the Shh+ endoderm.
TS17: oral region epithelium	Present	Regionally restricted	7C	Expression was located at the oral surface, but boundary cells co-expressing Cxcl14 remain broadly distributed.

Structure	Level	Pattern	Image	Note
TS18: oral region epithelium	Present	Regionally restricted	7D' lower left , 7D , 7D' , 7D' lower right	Expression was detected in the dental epithelium. Dmrt2-expressing cells in the posterior region delineate Irx1 staining in the dental lamina posteriorly. The anterior boundary was delineated with Cxcl14 expression.

Structure	Level	Pattern	Image	Note
TS20: mandibular process epithelium	Present	Regionally restricted	7E , 7E' , 7E''	Expression was detected in the dental epithelium. Some overlap with Cxcl14 expression was observed at the anterior boundary (2-4 cells) and with Dmrt2 expression at the posterior boundary.

Structure	Level	Pattern	Image	Note
TS18: oral region epithelium	Present	Regionally restricted	S9C E11.0 upper	Expression was detected in dental epithelium.

Structure	Level	Pattern	Image	Note
TS19: mandibular process	Present	Regionally restricted	S9C E11.5 upper	Expression was detected in dental epithelium.

Structure	Level	Pattern	Image	Note
TS20: mandibular process epithelium	Present	Regionally restricted	S9C E12.0 upper	Expression was detected in dental epithelium.

Structure	Level	Pattern	Image	Note
TS20: mandibular process epithelium	Present	Regionally restricted	S9C E12.5 upper	Expression was detected in dental epithelium.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	S10B left	Expression was detected in the majority of ectodermal cells in medial mandible region.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	S10C left	Expression was detected in the majority of ectodermal cells in lateral mandible region.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	S10D left	Expression was located at the oral surface.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	S10H right	Expression was detected ventrally in the newly formed mandibular arch epithelium at E8.5.