Gene Expression Data
Assay Details
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Assay
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Reference:
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J:320488
Tanimizu N, et al., Intrahepatic bile ducts are developed through formation of homogeneous continuous luminal network and its dynamic rearrangement in mice. Hepatology. 2016 Jul;64(1):175-88
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7614780
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Gene symbol:
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Spp1
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Gene name:
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secreted phosphoprotein 1
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Results
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Specimen
1B1/3:
postnatal week 14
(more  )
Note: Double labeled: green - Spp1; red - Pecam1 (assay MGI:7614781).
Specimen
1B1/3:
(close  )
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Genetic Background:
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C57BL/6
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Age:
|
postnatal week 14
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Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Spp1; red - Pecam1 (assay MGI:7614781).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: biliary ductule |
Present |
Regionally restricted |
1B1
,
1B3
|
Expression in large ducts and small ductules around portal vein.
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| TS28: intrahepatic bile duct |
Present |
Regionally restricted |
1B1
,
1B3
|
Expression in large ducts and small ductules around portal vein.
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Specimen
1E1/3:
postnatal week 1
(more )
Note: Double labeled: green - Spp1; red - Pecam1 (assay MGI:7614781).
Specimen
1E1/3:
(close )
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Genetic Background:
|
C57BL/6
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Age:
|
postnatal week 1
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Sex:
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Not Specified
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Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Spp1; red - Pecam1 (assay MGI:7614781).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
1E3
,
1E1
|
Expression in cholangiocytes forming network around portal vein. Luminal network similar to that of adult.
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| TS28: biliary ductule |
|
Present |
Regionally restricted |
1E1
,
1E3
|
Expression in large ducts and small ductules around portal vein.
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| TS28: intrahepatic bile duct |
|
Present |
Regionally restricted |
1E1
,
1E3
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Expression in large ducts and small ductules around portal vein.
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Specimen
2A1:
embryonic day 16.0
(more )
Specimen
2A1:
(close )
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Genetic Background:
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C57BL/6
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Age:
|
embryonic day 16.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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|
Sex:
|
Not Specified
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|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
2A1
|
Expression in cholangiocytes forming mesh-like network around portal vein.
|
Specimen
2A2:
embryonic day 16.0
(more )
Specimen
2A2:
(close )
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Genetic Background:
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C57BL/6
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Age:
|
embryonic day 16.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
2A2
|
Network of cholangiocytes is interrupted at some points.
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Specimen
2A3:
embryonic day 16.0
(more )
Specimen
2A3:
(close )
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Genetic Background:
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C57BL/6
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Age:
|
embryonic day 16.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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|
Sex:
|
Not Specified
|
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Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
2A3
|
Expression in cholangiocytes at periphery, many not yet incorporated into network structure around portal vein.
|
Specimen
2B1:
embryonic day 17.0
(more )
Specimen
2B1:
(close )
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Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
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Sex:
|
Not Specified
|
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Type:
|
optical section
|
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Fixation:
|
4% Paraformaldehyde
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Specimen
2B2:
embryonic day 17.0
(more )
Specimen
2B2:
(close )
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Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
2B2
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Tubular network of cholangiocytes is more continuous compared to E16.
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Specimen
2B3:
embryonic day 17.0
(more )
Specimen
2B3:
(close )
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Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
2B3
|
Expression in cholangiocytes at periphery, many not yet incorporated into network structure around portal vein.
|
Specimen
S2A2:
embryonic day 17.0
(more )
Note: Triple labeled: green - Spp1; white - Pecam1 (assay MGI:7614781); red - Tjp1 (assay MGI:7614782).
Specimen
S2A2:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
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Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Spp1; white - Pecam1 (assay MGI:7614781); red - Tjp1 (assay MGI:7614782).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S2A2
|
Expression in cholangiocytes in region where intrahepatic bile ducts form continuous network.
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Specimen
S2A3/5:
embryonic day 17.0
(more )
Note: Double labeled: green - Spp1; red - Tjp1 (assay MGI:7614782).
Specimen
S2A3/5:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
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Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Spp1; red - Tjp1 (assay MGI:7614782).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S2A5
,
S2A3
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Expression in cholangiocytes forming continuous network associated with tight junction network.
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Specimen
S2B2:
embryonic day 17.0
(more )
Note: Triple labeled: green - Spp1; white - Pecam1 (assay MGI:7614781); red - Tjp1 (assay MGI:7614782).
Specimen
S2B2:
(close )
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Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 17.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Spp1; white - Pecam1 (assay MGI:7614781); red - Tjp1 (assay MGI:7614782).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: biliary tract |
Present |
Regionally restricted |
S2B2
|
Expression in peripheral region of liver.
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Specimen
S2B3/5:
embryonic day 17.0
(more )
Note: Double labeled: green - Spp1; red - Tjp1 (assay MGI:7614782).
Specimen
S2B3/5:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Spp1; red - Tjp1 (assay MGI:7614782).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S2B5
,
S2B3
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Expression in interconnected cholangiocytes, interrupted where cholangiocytes are not yet differentiated.
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Specimen
5C1:
embryonic day 17.0
(more )
Note: Triple labeled: green - Spp1; white - Hnf4a (assay MGI:7614783); red - Tjp1 (assay MGI:7614782).
Specimen
5C1:
(close )
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Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Spp1; white - Hnf4a (assay MGI:7614783); red - Tjp1 (assay MGI:7614782).
|
Specimen
5C2:
embryonic day 18.0
(more )
Note: Triple labeled: green - Spp1; white - Hnf4a (assay MGI:7614783); red - Tjp1 (assay MGI:7614782).
Specimen
5C2:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Spp1; white - Hnf4a (assay MGI:7614783); red - Tjp1 (assay MGI:7614782).
|
Specimen
5C3:
postnatal week 1
(more )
Note: Triple labeled: green - Spp1; white - Hnf4a (assay MGI:7614783); red - Tjp1 (assay MGI:7614782).
Specimen
5C3:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal week 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Spp1; white - Hnf4a (assay MGI:7614783); red - Tjp1 (assay MGI:7614782).
|
Specimen
S6A1:
embryonic day 18.0
(more )
Specimen
S6A1:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S6A1
|
Expression in cholangiocytes forming 3D mesh-like network structures.
|
Specimen
S6A2:
embryonic day 18.0
(more )
Specimen
S6A2:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S6A2
|
Expression in cholangiocytes forming 3D mesh-like network structures.
|
Specimen
S6A3/4:
embryonic day 18.0
(more )
Note: Double labeled: green - Spp1; magenta - Pecam1 (assay MGI:7614781).
Specimen
S6A3/4:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Spp1; magenta - Pecam1 (assay MGI:7614781).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S6A4
,
S6A3
|
Expression in layer of cholangiocytes surrounding portal vein, as if expressing cells form the ductal plate.
|
Specimen
S6A5:
postnatal adult
(more )
Specimen
S6A5:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S6A5
|
Expression in cholangiocytes forming network structures.
|
Specimen
S6A6:
postnatal adult
(more )
Specimen
S6A6:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S6A6
|
Expression in cholangiocytes forming network structures.
|
Specimen
S6A7/8:
postnatal adult
(more )
Note: Double labeled: green - Spp1; magenta - Pecam1 (assay MGI:7614781).
Specimen
S6A7/8:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Spp1; magenta - Pecam1 (assay MGI:7614781).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: biliary ductule |
Present |
Regionally restricted |
S6A8
,
S6A7
|
Expression in a large duct and some small ductules around the portal vein.
|
| TS28: intrahepatic bile duct |
Present |
Regionally restricted |
S6A8
,
S6A7
|
Expression in a large duct and some small ductules around the portal vein.
|
Specimen
S7A:
embryonic day 16.0
(more )
Note: Double labeled: green - Spp1; red - Krt19 (assay MGI:7614784).
Specimen
S7A:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Spp1; red - Krt19 (assay MGI:7614784).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S7A
|
Expression in cholangiocytes forming biliary network
|
Specimen
S7A a-1/3:
embryonic day 16.0
(more )
Note: Double labeled: green - Spp1; red - Krt19 (assay MGI:7614784).
Specimen
S7A a-1/3:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Spp1; red - Krt19 (assay MGI:7614784).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S7A a-3
,
S7A a-1
|
Expression in cholangiocytes forming biliary network
|
Specimen
S7A b-1/3:
embryonic day 16.0
(more )
Note: Double labeled: green - Spp1; red - Krt19 (assay MGI:7614784).
Specimen
S7A b-1/3:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Spp1; red - Krt19 (assay MGI:7614784).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S7A b-1
,
S7A b-3
|
Expression in cholangiocytes forming biliary network
|
Specimen
S7B1/3/4/6:
postnatal week 1
(more )
Note: Double labeled: green - Spp1; red - Krt19 (assay MGI:7614784).
Specimen
S7B1/3/4/6:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal week 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Spp1; red - Krt19 (assay MGI:7614784).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S7B1
,
S7B3
,
S7B4
,
S7B6
|
Expression in cholangiocytes in peripheral as well as hilum of liver.
|
Specimen
S7B7/9/10/12:
postnatal week 8
(more )
Note: Double labeled: green - Spp1; red - Krt19 (assay MGI:7614784).
Specimen
S7B7/9/10/12:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal week 8
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Spp1; red - Krt19 (assay MGI:7614784).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: biliary tract |
cholangiocyte |
Present |
Regionally restricted |
S7B10
,
S7B7
,
S7B12
,
S7B9
|
Expression in cholangiocytes in peripheral as well as hilum of liver.
|
|
Analysis Tools