Probe:	GUDMAP Dkk1 probe 2178
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS19: paramesonephric duct	Present		1B 11.5 XY Dorsal
TS19: mesonephric tubule	Present		1B 11.5 XY Dorsal
TS19: gonad	Weak		1B 11.5 XY Dorsal

Structure	Level	Pattern	Image	Note
TS19: mesonephric tubule	Present		1B 11.5 XY Ventral
TS19: paramesonephric duct	Present		1B 11.5 XY Ventral
TS19: gonad	Weak		1B 11.5 XY Ventral

Structure	Level	Pattern	Image	Note
TS19: gonad	Absent		1B 11.5 XX Dorsal	No expression in the female gonads.
TS19: mesonephric tubule	Present		1B 11.5 XX Dorsal	Similar as in the male.
TS19: paramesonephric duct	Present		1B 11.5 XX Dorsal	Similar as in the male.

Structure	Level	Pattern	Image	Note
TS19: gonad	Absent		1B 11.5 XX Ventral	No expression in the female gonads.
TS19: mesonephric tubule	Present		1B 11.5 XX Ventral	Similar as in the male.
TS19: paramesonephric duct	Present		1B 11.5 XX Ventral	Similar as in the male.

Structure	Level	Pattern	Image	Note
TS20: coelomic epithelium of testis	Present		1B 12.5 XY Dorsal
TS20: mesonephric tubule of male	Present		1B 12.5 XY Dorsal
TS20: testis	Present	Regionally restricted	1B 12.5 XY Dorsal	Expressed predominantly under the coelomic epithelium.
TS20: paramesonephric duct	Present	Regionally restricted	1B 12.5 XY Dorsal	Expression was restricted to the dorsal end, more severely in the male compared with the female.

Structure	Level	Pattern	Image	Note
TS20: coelomic epithelium of testis	Present		1B 12.5 XY Ventral
TS20: mesonephric tubule of male	Present		1B 12.5 XY Ventral
TS20: testis	Present	Regionally restricted	1B 12.5 XY Ventral	Expressed predominantly under the coelomic epithelium.
TS20: paramesonephric duct	Present	Regionally restricted	1B 12.5 XY Ventral	Expression was restricted to the dorsal end, more severely in the male compared with the female.

Structure	Level	Pattern	Image	Note
TS20: ovary	Absent		1B 12.5 XX Dorsal
TS20: paramesonephric duct	Present	Regionally restricted	1B 12.5 XX Dorsal	Expression was restricted to the dorsal end.
TS20: mesonephric tubule of female	Present		1B 12.5 XX Dorsal

Structure	Level	Pattern	Image	Note
TS20: ovary	Absent		1B 12.5 XX Ventral
TS20: paramesonephric duct	Present	Regionally restricted	1B 12.5 XX Ventral	Expression was restricted to the dorsal end.
TS20: mesonephric tubule of female	Present		1B 12.5 XX Ventral

Structure	Level	Pattern	Image	Note
TS21: testis	Present		1B 13.5 XY Dorsal	Expression expanded to encompass the poles.
TS21: mesenchyme of paramesonephric duct of male	Present		1B 13.5 XY Dorsal
TS21: mesonephros of male	Absent		1B 13.5 XY Dorsal

Structure	Level	Pattern	Image	Note
TS21: testis	Present		1B 13.5 XY Ventral	Expression expanded to encompass the poles.
TS21: mesenchyme of paramesonephric duct of male	Present		1B 13.5 XY Ventral
TS21: mesonephros of male	Absent		1B 13.5 XY Ventral

Structure	Level	Pattern	Image	Note
TS21: ovary	Present	Regionally restricted	1B 13.5 XX Dorsal	Expressed in a single focal point in the posterior region.
TS21: mesonephric tubule of female	Present		1B 13.5 XX Dorsal

Structure	Level	Pattern	Image	Note
TS21: ovary	Present	Regionally restricted	1B 13.5 XX Ventral	Expressed in a single focal point in the posterior region.