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Gene Expression Data
Assay Details
Assay
Reference: J:342644 Tscherner AK, et al., Relationship of quantitative reverse transcription polymerase chain reaction (RT-PCR) to RNA Sequencing (RNAseq) transcriptome identifies mouse preimplantation embryo reference genes. Biol Reprod. 2023 Nov 15;109(5):601-617
Assay type: RT-PCR
MGI Accession ID: MGI:7614302
Gene symbol: Polr2f
Gene name: polymerase (RNA) II (DNA directed) polypeptide F
Probe: Polr2f-pF, Polr2f-pR
Probe preparation: labelled with SYBR green
Assay notes: Nested RT-PCR, the outer primer set was: MGI:7612139. Quantitative RT-PCR. Relative expression was normalized to an internal control plasmid.
Results Image: 5A
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
GV P w 5-8 TS28: primary oocyte Present Not Specified; total RNA CD-1 Female
MII P w 5-8 TS28: secondary oocyte Present Not Specified; total RNA CD-1 Female
1c E0.5 TS1: 1-cell stage conceptus Present Not Specified; total RNA CD-1 Not Specified 1-cell stage conceptus.
2c E1.0 TS2: 2-cell stage conceptus Present Not Specified; total RNA CD-1 Not Specified 2-cell stage conceptus.
4c E2.0 TS3: 4-cell stage conceptus Present Not Specified; total RNA CD-1 Not Specified 4-cell stage conceptus.
8c E2.5 TS3: 8-cell stage conceptus Present Not Specified; total RNA CD-1 Not Specified 8-cell stage conceptus.
M E3.0 TS3: morula-stage conceptus Present Not Specified; total RNA CD-1 Not Specified Morula stage conceptus.
Bl E3.5 TS4: blastocyst-stage conceptus Present Not Specified; total RNA CD-1 Not Specified Blastocyst stage conceptus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory