Probe:	ChM-I cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: cartilage condensation	Present	Regionally restricted	3A	Expression was detected in cartilaginous bone rudiments. Expression was detected in proliferating and mature cartilage zones.
TS21: chondrocranium pre-cartilage condensation	Present	Regionally restricted	3A	Expression was found in the cartilaginous rudiment of the occipital bone.
TS21: vertebral cartilage condensation	Present		3B , 3A
TS21: rib cartilage condensation	Present		3A
TS21: trachea	Present	Regionally restricted	3A	Expression was detected in tracheal rings.
TS21: notochord	Absent		3A , 3B

Structure	Level	Pattern	Image	Note
TS21: hindlimb cartilage condensation	Present		3C

Structure	Cell Type	Level	Pattern	Image	Note
TS24: vertebra		Present	Non-Uniform	4A	A high level of expression was detected from the proliferative to the early hypertrophic zone of cartilage. The mRNA expression was completely abolished in the late hypertrophic and calcified zone.
TS24: vertebra	hypertrophic chondrocyte	Weak	Regionally restricted	4A	Expression of transcripts declined in the late hypertrophic chondrocytes at the center of vertebral columns.

Structure	Level	Pattern	Image	Note
TS24: Meckel's cartilage	Present	Regionally restricted	4B	A high level of expression was detected from the proliferative to the early hypertrophic zone of cartilage. The mRNA expression was completely abolished in the late hypertrophic and calcified zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: cranium	preosteoblast	Weak	Regionally restricted	4C	Expression was detected in immature osteoblasts in the dermocranium.
TS21: chondrocranium pre-cartilage condensation		Present		4C