Nefh Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:145300 Kim AY, et al., Pirt, a phosphoinositide-binding protein, functions as a regulatory subunit of TRPV1. Cell. 2008 May 2;133(3):475-85
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7611627
Gene symbol:	Nefh
Gene name:	neurofilament, heavy polypeptide

Antibody:	Anti-NF200 (AB1982)
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Results

Specimen S3C: postnatal adult; Pirt^tm1Xzd/Pirt⁺ (more

)
Note: L4-L6 lumbar region dorsal root ganglion. Double labeled: red - Nefh; green - Pirt reporter (assay MGI:7611628).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dorsal root ganglion	sensory neuron of dorsal root ganglion	Present		S3C	Immunoreactivity was detected in sensory neurons. Double labeled cells were observed.

Specimen not shown +/+: postnatal adult (more

)
Note: Data from Table in 2C.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dorsal root ganglion	sensory neuron of dorsal root ganglion	Present

Specimen not shown -/-: postnatal adult; Pirt^tm1Xzd/Pirt^tm1Xzd (more

)
Note: Data from Table in 2C.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dorsal root ganglion	sensory neuron of dorsal root ganglion	Present			The mutant had normal proportions of immunostained neurons.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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