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Gene Expression Data
Assay Details
Assay
Reference: J:168645 Xavier GM, et al., Characterization of a mouse Scube3 reporter line. Genesis. 2010 Dec;48(12):684-92
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7611604
Gene symbol: Scube3
Gene name: signal peptide, CUB domain, EGF-like 3
Reporter: lacZ
Direct Detection
Results
Specimen 1a: embryonic day 9.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS15: 1st branchial arch ectoderm Strong 1a
TS15: 2nd branchial arch ectoderm Strong 1a
TS15: facial prominence ectoderm Strong 1a
TS15: fronto-nasal process ectoderm Strong 1a
TS15: nasal process ectoderm Strong 1a
TS15: otocyst epithelium Strong 1a
TS15: limb Present 1a
TS15: neural tube Present 1a
Specimen 1b: embryonic day 9.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS15: 1st branchial arch ectoderm Strong 1b
TS15: 2nd branchial arch ectoderm Strong 1b
TS15: facial prominence ectoderm Strong 1b
TS15: fronto-nasal process ectoderm Strong 1b
TS15: nasal process ectoderm Strong 1b
TS15: otocyst epithelium Strong 1b
TS15: limb Present 1b
TS15: neural tube Present 1b
Specimen 1c: embryonic day 9.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS15: 1st branchial arch ectoderm Strong 1c
TS15: 2nd branchial arch ectoderm Strong 1c
TS15: facial prominence ectoderm Strong 1c
TS15: fronto-nasal process ectoderm Strong 1c
TS15: nasal process ectoderm Strong 1c
TS15: otocyst epithelium Strong 1c
TS15: limb Present 1c
TS15: neural tube Present 1c
Specimen 1d: embryonic day 10.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS17: apical ectodermal ridge Present 1d
TS17: nasal pit epithelium Present 1d
TS17: nasal process ectoderm Present 1d
TS17: otocyst epithelium Present 1d
TS17: 1st branchial arch maxillary component ectoderm Present Regionally restricted 1d Expression is extending into the midline of the fronto-nasal and maxillary processes.
TS17: facial prominence ectoderm Present Regionally restricted 1d Expression is extending into the midline of the fronto-nasal and maxillary processes.
Specimen 1e: embryonic day 10.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS17: apical ectodermal ridge Present 1e
TS17: nasal pit epithelium Present 1e
TS17: nasal process ectoderm Present 1e
TS17: otocyst epithelium Present 1e
TS17: 1st branchial arch maxillary component ectoderm Present Regionally restricted 1e Expression is extending into the midline of the fronto-nasal and maxillary processes.
TS17: facial prominence ectoderm Present Regionally restricted 1e Expression is extending into the midline of the fronto-nasal and maxillary processes.
Specimen 1f: embryonic day 10.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS17: apical ectodermal ridge Present 1f
TS17: nasal pit epithelium Present 1f
TS17: nasal process ectoderm Present 1f
TS17: otocyst epithelium Present 1f
TS17: 1st branchial arch maxillary component ectoderm Present Regionally restricted 1f Expression is extending into the midline of the fronto-nasal and maxillary processes.
TS17: facial prominence ectoderm Present Regionally restricted 1f Expression is extending into the midline of the fronto-nasal and maxillary processes.
Specimen 1g: embryonic day 10.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS17: nasal pit epithelium Present 1g
TS17: nasal process ectoderm Present 1g
TS17: face mesenchyme Weak 1g
Specimen 1h: embryonic day 10.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS17: nasal pit epithelium Present 1h
TS17: 1st branchial arch maxillary component ectoderm Present Regionally restricted 1h Expression is extending into the midline of the fronto-nasal and maxillary processes.
TS17: face mesenchyme Weak 1h
Specimen 1i: embryonic day 10.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS17: 1st branchial arch maxillary component ectoderm Present 1i
TS17: facial prominence ectoderm Present 1i
TS17: nasal pit epithelium Present 1i
TS17: face mesenchyme Weak 1i
Specimen 1j: embryonic day 10.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS17: otocyst epithelium Present 1j
TS17: face mesenchyme Weak 1j
Specimen 1k: embryonic day 10.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS17: apical ectodermal ridge Present 1k
TS17: limb mesenchyme Weak 1k
Specimen 1l: embryonic day 11.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present 1l
TS19: otocyst epithelium Present 1l
TS19: facial prominence ectoderm Present Regionally restricted 1l Expression is in the fusing facial processes.
TS19: neural tube Present Regionally restricted 1l Expression is ventral.
Specimen 1m: embryonic day 11.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present 1m
TS19: otocyst epithelium Present 1m
TS19: facial prominence ectoderm Present Regionally restricted 1m Expression is in the fusing facial processes.
TS19: neural tube Present Regionally restricted 1m Expression is ventral.
TS19: dermomyotome Present Regionally restricted 1m Expression is in the ventral and dorsal dermomyotome.
Specimen 1n: embryonic day 11.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present 1n
TS19: neural tube Present Regionally restricted 1n Expression is ventral.
Specimen 2a: embryonic day 12.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS20: palatal shelf epithelium Present 2a
TS20: tooth Present 2a
TS20: vibrissa Present 2a
TS20: eye Present Regionally restricted 2a Expression is in the optic eminence.
TS20: nose Present Regionally restricted 2a Expression is in the external nares.
TS20: lip Present Regionally restricted 2a Expression is in the lip philtrum.
Specimen 2b: embryonic day 12.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS20: palatal shelf epithelium Present 2b
TS20: tooth Present 2b
TS20: vibrissa Present 2b
TS20: eye Present Regionally restricted 2b Expression is in the optic eminence.
TS20: nose Present Regionally restricted 2b Expression is in the external nares.
TS20: lip Present Regionally restricted 2b Expression is in the lip philtrum.
Specimen 2c: embryonic day 13.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS21: external naris Present 2c
TS21: lower jaw incisor Present 2c
TS21: lower jaw molar Present 2c
TS21: palatal rugae Present 2c
TS21: palatal shelf epithelium Present 2c
TS21: philtrum Present 2c
TS21: upper jaw incisor Present 2c
TS21: upper jaw molar Present 2c
TS21: vibrissa Present 2c
TS21: eye Present Regionally restricted 2c Expression is in the optic eminence.
Specimen 2d: embryonic day 13.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS21: external naris Present 2d
TS21: lower jaw incisor Present 2d
TS21: lower jaw molar Present 2d
TS21: palatal rugae Present 2d
TS21: palatal shelf epithelium Present 2d
TS21: philtrum Present 2d
TS21: upper jaw incisor Present 2d
TS21: upper jaw molar Present 2d
TS21: vibrissa Present 2d
TS21: eye Present Regionally restricted 2d Expression is in the optic eminence.
Specimen 3a: embryonic day 12.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS20: tooth bud Strong 3a
TS20: palatal shelf epithelium Present 3a
TS20: palatal shelf Present Regionally restricted 3a Expression is in the medial epithelial seam between the palatal shelves.
TS20: upper lip Present Regionally restricted 3a Expression is in the lip philtrum.
Specimen 3b: embryonic day 13.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS21: palatal rugae Strong 3b
TS21: upper jaw incisor Strong 3b
TS21: upper jaw molar Strong 3b
TS21: palatal shelf Present Regionally restricted 3b Expression is in the medial epithelial seam between the palatal shelves.
Specimen 3c: embryonic day 14.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS22: palatal rugae Strong 3c
TS22: upper jaw tooth Strong 3c
TS22: palatal shelf Present Regionally restricted 3c Expression is in the medial epithelial seam between the palatal shelves.
Specimen 3d: embryonic day 12.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS20: tooth Strong 3d
Specimen 3e: embryonic day 13.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS21: lower jaw incisor Strong 3e
TS21: lower jaw molar Strong 3e
Specimen 3f: embryonic day 14.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS22: lower jaw tooth Strong 3f
TS22: fungiform papillae Present 3f
Specimen 4a: embryonic day 13.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS21: incisor Present 4a
TS21: nasal epithelium Present 4a
Specimen 4b: embryonic day 13.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS21: incisor Present 4b
TS21: molar Present 4b
TS21: nasal epithelium Present 4b
Specimen 4c: embryonic day 14.5; Scube3tm1Dgen/Scube3+ (more )
Note: Cap stage of tooth development. Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS22: cervical loop Present 4c
TS22: enamel knot Present 4c
TS22: inner enamel epithelium Present 4c
TS22: oral epithelium Present 4c
TS22: outer enamel epithelium Present 4c
Specimen 4d: embryonic day 16.5; Scube3tm1Dgen/Scube3+ (more )
Note: Bell stage of tooth development. Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Cell Type Level Pattern Image Note
TS24: stellate reticulum Present 4d
TS24: inner enamel epithelium preameloblast Present 4d Expression is in differentiated cell populations including preameloblasts.
Specimen 4e: embryonic day 14.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS22: vibrissa dermal component Present 4e
TS22: vibrissa hair Present 4e
TS22: vibrissa root sheath Present Regionally restricted 4e Expression is in the outer root sheath.
Specimen 4f: embryonic day 13.5; Scube3tm1Dgen/Scube3+ (more )
Note: Fixative was 2% formaldehyde; 0.2% gluteraldehyde.
Structure Level Pattern Image Note
TS21: hyaloid vascular plexus Present 4f
TS21: lens fiber Present 4f
TS21: neural retina Present 4f
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory