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Gene Expression Data
Assay Details
Assay
Reference: J:344337 Mishra A, et al., Atrial natriuretic peptide signaling co-regulates lipid metabolism and ventricular conduction system gene expression in the embryonic heart. iScience. 2024 Jan 19;27(1):108748
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7610930
Gene symbol: Nppa
Gene name: natriuretic peptide type A
Antibody: Anti-Atrial Natriuretic Peptide Antibody, clone A6 (CBL66)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 1B/D: embryonic day 11.5 (more )
Note: Double labeled: red - Nppa; green - Npr1 (assay MGI:7610931).
Structure Level Pattern Image Note
TS19: left ventricle cardiac muscle Present Regionally restricted 1D , 1B The percentage of positive cells was 8.4-fold higher in the trabecular myocardium compared to the compact myocardium (64% vs. 7.6%, summarized in Fig 1H).

Specimen 1L/N: embryonic day 11.5 (more )
Note: Double labeled: red - Nppa; green - Gja5 (assay MGI:7610933).
Structure Level Pattern Image Note
TS19: left ventricle cardiac muscle Present 1L , 1N Coexpressed with Gja5 in many cell clusters in the trabecular myocardium.

Specimen 3B/C: embryonic day 11.5 (more )
Note: Double labeled: red - Nppa; green - BODIPY.
Structure Level Pattern Image Note
TS19: ventricle cardiac muscle Present 3B , 3C Costained with lipid droplets dye (BODIPY) in the trabecular myocardium.

Specimen S1C/D: embryonic day 11.5 (more )
Note: Double labeled: red - Nppa; green - Npr1 (assay MGI:7610931).
Structure Level Pattern Image Note
TS19: right ventricle cardiac muscle Present S1D , S1C The percentage of positive cells was significant higher in the trabecular myocardium compared to the compact myocardium.
TS19: interventricular septum cardiac muscle Present S1C , S1D The percentage of positive cells was significant higher compared to the compact myocardium.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory