Gene Expression Data
Assay Details
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Assay
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Reference:
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J:173957
Tsutsumi M, et al., Characterization of a Novel Mouse Gene Encoding an SYCP3-Like Protein That Relocalizes from the XY Body to the Nucleolus During Prophase of Male Meiosis I. Biol Reprod. 2011 Jul;85(1):165-71
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7610717
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Gene symbol:
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H2ax
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Gene name:
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H2A.X variant histone
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Antibody:
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anti-gammaH2AX
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Detection system:
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Secondary antibody coupled to other - see notes
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Results
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Specimen
3A Spermatocytes Pachytene:
postnatal adult
(more  )
Note: Meiotic nuclear spreads, with aminomethylcoumarin acetate-conjugated secondary antibody. Triple labeled: blue - H2ax; green - 1700013H16Rik (assay MGI:7610715); red - Sycp3 (assay MGI:7610716).
Specimen
3A Spermatocytes Pachytene:
(close  )
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Genetic Background:
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DDY
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Meiotic nuclear spreads, with aminomethylcoumarin acetate-conjugated secondary antibody. Triple labeled: blue - H2ax; green - 1700013H16Rik (assay MGI:7610715); red - Sycp3 (assay MGI:7610716).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
3A sperm Pach merge
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Expressed in the the XY body.
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Analysis Tools
