Probe:	LH2 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present		2B
TS11: extraembryonic component	Absent		2B	No significant expression in extraembrynic tissues at this stage.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	2E	Expression in tailbud.
TS13: branchial arch	Present		2E	Expression in somites, in region of dorsal aorta, branchial arches, hindbrain and forebrain.
TS13: dorsal aorta	Present		2E	Expression in somites, in region of dorsal aorta, branchial arches, hindbrain and forebrain.
TS13: future hindbrain	Present		2E	Expression in somites, in region of dorsal aorta, branchial arches, hindbrain and forebrain.
TS13: future prosencephalon	Present		2E	Expression in somites, in region of dorsal aorta, branchial arches, hindbrain and forebrain.
TS13: somite	Present		2E	Expression in somites, in region of dorsal aorta, branchial arches, hindbrain and forebrain.
TS13: 1st branchial arch	Present	Regionally restricted	2E	Expression particularly in mandibular and maxillary components of first branchial arch.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		2H	Expression in tailbud, somites, in region of dorsal aorta, branchial arches, hindbrain and forebrain.
TS15: dorsal aorta	Present		2H	Expression in tailbud, somites, in region of dorsal aorta, branchial arches, hindbrain and forebrain.
TS15: future forebrain	Present		2H	Expression in tailbud, somites, in region of dorsal aorta, branchial arches, hindbrain and forebrain.
TS15: future hindbrain	Present		2H	Expression in tailbud, somites, in region of dorsal aorta, branchial arches, hindbrain and forebrain.
TS15: somite	Present		2H	Expression in tailbud, somites, in region of dorsal aorta, branchial arches, hindbrain and forebrain.
TS15: tail	Present		2H	Expression in tailbud, somites, in region of dorsal aorta, branchial arches, hindbrain and forebrain.
TS15: 1st branchial arch mandibular component	Present		2H	Expression particularly in mandibular and maxillary components of first branchial arch.
TS15: 1st branchial arch maxillary component	Present		2H	Expression particularly in mandibular and maxillary components of first branchial arch.
TS15: limb bud	Present		2H	Expression in limb buds.