Gene Expression Data
Assay Details
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Assay
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Reference:
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J:286599
Song NN, et al., Rnf220/Zc4h2-mediated monoubiquitylation of Phox2 is required for noradrenergic neuron development. Development. 2020 Mar 30;147(6):dev185199
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7608298
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Gene symbol:
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Dbh
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Gene name:
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dopamine beta hydroxylase
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Results
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Specimen
1A Dbh:
embryonic day 10.5
(more  )
Specimen
1A Dbh:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 1 |
Present |
Regionally restricted |
1A Dbh
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Expression in dorsal rhombomere 1.
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Specimen
1A Dbh/Phox2a:
embryonic day 10.5
(more )
Note: Double labeled: blue - Dbh; brown - Phox2a (assay MGI:7608327).
Specimen
1A Dbh/Phox2a:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Dbh; brown - Phox2a (assay MGI:7608327).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 1 |
Present |
Regionally restricted |
1A Dbh/Phox2a
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Expression in dorsal rhombomere 1, colocalized with Phox2a expression.
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Specimen
1A Dbh/Phox2b:
embryonic day 10.5
(more )
Note: Double labeled: blue - Dbh; brown - Phox2b (assay MGI:7608329).
Specimen
1A Dbh/Phox2b:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Dbh; brown - Phox2b (assay MGI:7608329).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 1 |
Present |
Regionally restricted |
1A Dbh/Phox2b
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Expression in dorsal rhombomere 1, colocalized with Phox2b expression.
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Specimen
1B Dbh/Phox2a:
embryonic day 12.5
(more )
Note: Double labeled: blue - Dbh; brown - Phox2a (assay MGI:7608327).
Specimen
1B Dbh/Phox2a:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Dbh; brown - Phox2a (assay MGI:7608327).
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Specimen
1B Dbh/Phox2b:
embryonic day 12.5
(more )
Note: Double labeled: blue - Dbh; brown - Phox2b (assay MGI:7608329).
Specimen
1B Dbh/Phox2b:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Dbh; brown - Phox2b (assay MGI:7608329).
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Specimen
1B Dbh/Zc4h2:
embryonic day 12.5
(more )
Note: Double labeled: blue - Dbh; brown - Zc4h2 (assay MGI:7608331).
Specimen
1B Dbh/Zc4h2:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Dbh; brown - Zc4h2 (assay MGI:7608331).
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Specimen
2C Dbh Control:
embryonic day 10.5
(more )
Specimen
2C Dbh Control:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2C Dbh Zc4h2 KO:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Zc4h2em1.1Yqd/Zc4h2em1.1Yqd
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 1 |
Present |
Regionally restricted |
2C Dbh KO
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Expression not changed compared to control.
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Specimen
2D Dbh Control:
embryonic day 12.5
(more )
Specimen
2D Dbh Control:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2D Dbh Zc4h2 KO:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Zc4h2em1.1Yqd/Zc4h2em1.1Yqd
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pons |
Trace |
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2D Dbh KO
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Expression significantly reduced compared to control.
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Specimen
2E Dbh Control:
embryonic day 16.5
(more )
Specimen
2E Dbh Control:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2E Dbh Zc4h2 KO:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Zc4h2em1.1Yqd/Zc4h2em1.1Yqd
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3A Dbh:
embryonic day 10.5
(more )
Specimen
3A Dbh:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 1 |
Present |
Regionally restricted |
3A Dbh
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Expression in dorsal rhombomere 1 overlapping with Rnf220 expression.
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Specimen
3B Dbh:
embryonic day 12.5
(more )
Specimen
3B Dbh:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pons |
Present |
Regionally restricted |
3B Dbh
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Expression overlapping with Rnf220 expression.
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Specimen
3C Dbh:
embryonic day 16.5
(more )
Specimen
3C Dbh:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3E Dbh Control:
embryonic day 10.5
(more )
Specimen
3E Dbh Control:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3E Dbh Rnf220 KO:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Rnf220em1.1Kust/Rnf220em1.1Kust
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3F Dbh Control:
embryonic day 12.5
(more )
Specimen
3F Dbh Control:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3F Dbh Rnf220 IO:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Rnf220em1.1Kust/Rnf220em1.1Kust
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pons |
Weak |
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3F Dbh KO
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Expression reduced compared to control.
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Specimen
3H Dbh Control:
embryonic day 16.5
(more )
Specimen
3H Dbh Control:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3H Dbh Rnf220 KO:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Rnf220em1.1Kust/Rnf220em1.1Kust
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S1A:
embryonic day 11.5
(more )
Specimen
S1A:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S2D Control:
embryonic day 12.5
(more )
Specimen
S2D Control:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S2D Zc4h2+/-:
embryonic day 12.5; Zc4h2em1.1Yqd/Zc4h2 +
(more )
Specimen
S2D Zc4h2+/-:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Zc4h2em1.1Yqd/Zc4h2+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools