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Gene Expression Data
Assay Details
Assay
Reference: J:166176 Zhou Y, et al., Activation of paternally expressed genes and perinatal death caused by deletion of the Gtl2 gene. Development. 2010 Aug;137(16):2643-52
Assay type: RT-PCR
MGI Accession ID: MGI:7607179
Gene symbol: Mir127
Gene name: microRNA 127
Probe: Mir127-pF, Mir127-pR
Assay notes: Quantitative RT-PCR. Relative expression was normalized to SnoRNA202. Values from wild-type embryos were set to 1.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
+/+ E16.5 TS24: embryo Present 0.01 µg; total RNA involves: 129S6/SvEvTac * C57BL/6J Not Specified
KO/+ E16.5 TS24: embryo Weak (a) 0.01 µg; total RNA B6.129S6-Meg3tm1Itl Meg3tm1Itl/Meg3+Not Specified Maternal (KO/+) deletion of the Gtl2 gene.
+/KO E16.5 TS24: embryo Present 0.01 µg; total RNA B6.129S6-Meg3tm1Itl Meg3tm1Itl/Meg3+Not Specified Paternal (+/KO) deletion of the Gtl2 gene.
- Other lane(s) in blot assay different gene(s)
Notes:
(a) Expression was reduced to 3.5% of wild-type levels.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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