Gene Expression Data
Assay Details
Assay
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Reference:
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J:174498
Oldekamp J, et al., bHLH gene expression in the Emx2-deficient dentate gyrus reveals defective granule cells and absence of migrating precursors. Cereb Cortex. 2004 Sep;14(9):1045-58
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7606958
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Gene symbol:
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Tnc
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Gene name:
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tenascin C
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Probe:
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TenC probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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A signal amplification step (tyramide signal amplification) was used.
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Results
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Specimen
7A:
embryonic day 14.5
(more )
Specimen
7A:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS22: allocortex |
Present |
Regionally restricted |
7A
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Expressed in dentate neuroepithelium.
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Specimen
7B:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS22: hippocampus |
Absent |
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7B
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No expression in the dentate.
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Specimen
7C:
embryonic day 18.5
(more )
Specimen
7C:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: dentate gyrus |
Strong |
Widespread |
7C
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Expressed in the ventricular and subventricular layers and at every level of the primordium.
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Specimen
7D:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: dentate gyrus |
Present |
Regionally restricted |
7D
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Expressed only in the germinal zone and in very few cells of the radial migration stream in the mutant.
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