Gene Expression Data
Assay Details
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Assay
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Reference:
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J:174498
Oldekamp J, et al., bHLH gene expression in the Emx2-deficient dentate gyrus reveals defective granule cells and absence of migrating precursors. Cereb Cortex. 2004 Sep;14(9):1045-58
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7606956
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Gene symbol:
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Id3
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Gene name:
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inhibitor of DNA binding 3
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Probe:
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Id3 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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A signal amplification step (tyramide signal amplification) was used.
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Results
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Specimen
5A:
embryonic day 14.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hippocampus |
Absent |
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5A
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No expression in the dentate.
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: allocortex |
Present |
Regionally restricted |
5B
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A patch of premature expression in the dentate neuroepithelium was seen in the mutant.
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Specimen
5C:
embryonic day 15.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hippocampus |
Present |
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5C
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Expressed in the hippocampal germinal layers, particularly in the fimbrial neuroepithelium.
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| TS23: dentate gyrus primordium |
Present |
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5C
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Expressed in the germinal layers, and in few radially migrating cells.
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: dentate gyrus primordium |
Present |
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5D
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Expression was similar at this age in the mutant, but no expression in migrating cells.
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Specimen
5E:
embryonic day 18.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: dentate gyrus |
Strong |
Regionally restricted |
5E
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Expressed throughout the developing dentate gyrus subregions. Expressed in the ventricular layer as well as in cells along the migratory pathway. Expression increased distally, reaching a maximum in the tertiary matrix.
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: dentate gyrus |
Present |
Regionally restricted |
5F
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Expression was restricted to the germinal layer. No expression in the migratory stream, while the well-defined patch of expression in the germinal layers appeared wider and more intensely labeled than in the wild-type.
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Analysis Tools