Gene Expression Data
Assay Details
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Assay
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Reference:
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J:174498
Oldekamp J, et al., bHLH gene expression in the Emx2-deficient dentate gyrus reveals defective granule cells and absence of migrating precursors. Cereb Cortex. 2004 Sep;14(9):1045-58
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7606955
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Gene symbol:
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Hes5
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Gene name:
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hes family bHLH transcription factor 5
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Probe:
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Hes5 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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A signal amplification step (tyramide signal amplification) was used.
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Results
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Specimen
5G:
embryonic day 14.5
(more  )
Specimen
5G:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: allocortex |
Present |
Regionally restricted |
5G
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Expressed in the dentate neuroepithelium.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: allocortex |
Present |
Regionally restricted |
5H
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Expressed in the dentate neuroepithelium, similar as in the wild-type.
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Specimen
5I:
embryonic day 15.5
(more )
Specimen
5I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: dentate gyrus primordium |
Present |
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5I
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Expressed in the germinal layers and radially migrating cells. Abundant labeled cells formed a wide radial migration stream.
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Specimen
5J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: dentate gyrus primordium |
Present |
Regionally restricted |
5J
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Expressed in the dentate neuroepithelium, but no labeled migrating cells were seen.
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Specimen
5K:
embryonic day 18.5
(more )
Specimen
5K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: dentate gyrus |
Present |
Regionally restricted |
5K
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Expressed in every subregion. Expressed in ventricular zone, migratory stream and tertiary matrix. Expression intensity was the same through the primordium.
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Specimen
5L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: dentate gyrus |
Present |
Regionally restricted |
5L
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Expression was still confined to the ventricular zone in the mutant, no labeled migrating cells were seen.
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Analysis Tools