Gene Expression Data
Assay Details
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Assay
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Reference:
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J:174498
Oldekamp J, et al., bHLH gene expression in the Emx2-deficient dentate gyrus reveals defective granule cells and absence of migrating precursors. Cereb Cortex. 2004 Sep;14(9):1045-58
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7606953
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Gene symbol:
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Notch1
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Gene name:
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notch 1
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Probe:
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Notch1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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A signal amplification step (tyramide signal amplification) was used.
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Results
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Specimen
4A:
embryonic day 14.5
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: allocortex |
Weak |
Regionally restricted |
4A
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Expressed in dentate neuroepithelium.
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Specimen
4B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: allocortex |
Weak |
Regionally restricted |
4B
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Expressed in dentate neuroepithelium, similar as in the wild-type.
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Specimen
4C:
embryonic day 15.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: dentate gyrus primordium |
Strong |
Regionally restricted |
4C
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Intense expression in the ventricular layer and less intense in the subventricular. Labeled cells could be seen in the radial migratory stream as well as in the tangential stream, with a few cells scattered between dentate and Ammon's horn neuroepithelium.
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| TS23: hippocampus |
Present |
Regionally restricted |
4C
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Expressed in the hippocampal ventricular zone.
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Specimen
4D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hippocampus |
Present |
Regionally restricted |
4D
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Expressed in the germinal layers.
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| TS23: dentate gyrus primordium |
Weak |
Regionally restricted |
4D
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Expressed in the dentate neuroepithelium, but no labeled migrating cells were seen.
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Specimen
4E:
embryonic day 18.5
(more )
Specimen
4E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: dentate gyrus |
Present |
Widespread |
4E
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Expressed homogeneously in all subregions, from ventricular zone to tertiary matrix. Expressed in numerous cells in the migratory stream.
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Specimen
4F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: dentate gyrus |
Present |
Regionally restricted |
4F
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Expressed only in the mutant neuroepithelium, no labeled migrating cells were seen.
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| TS26: hippocampus |
Present |
Regionally restricted |
4F
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Expression in the mutant was restricted to the ventricular zone. No expression in the fimbrial neuroepithelium.
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