Gene Expression Data
Assay Details
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Assay
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Reference:
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J:174498
Oldekamp J, et al., bHLH gene expression in the Emx2-deficient dentate gyrus reveals defective granule cells and absence of migrating precursors. Cereb Cortex. 2004 Sep;14(9):1045-58
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7606952
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Gene symbol:
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Ascl1
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Gene name:
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achaete-scute family bHLH transcription factor 1
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Probe:
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Mash1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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A signal amplification step (tyramide signal amplification) was used.
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Results
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Specimen
3A:
embryonic day 14.5
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: allocortex |
Present |
Regionally restricted |
3A
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Expression formed a patch in the dentate neuroepithelium.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
3C:
embryonic day 15.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hippocampus |
Present |
Regionally restricted |
3C
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Expressed in the fimbrial neuroepithelium.
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| TS23: basal ganglia |
Present |
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3C
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| TS23: dentate gyrus primordium |
Present |
Regionally restricted |
3C
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Expressed in the neuroepithelium immediately adjacent to the fimbria and a few radially migrating cells starting to form the secondary matrix.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: dentate gyrus primordium |
Present |
Regionally restricted |
3D
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A smaller but correctly placed patch at the very end of the primordium was seen, compared with the wild-type. No cells were detected migrating from it.
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| TS23: hippocampus |
Present |
Regionally restricted |
3D
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A smaller but correctly placed patch at the very end of the primordium was seen, compared with the wild-type. No cells were detected migrating from it.
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| TS23: basal ganglia |
Present |
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3D
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Specimen
3E:
embryonic day 18.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Analysis Tools