Tg(Gtf2ird1)KrnJvvw
Transgene Detail
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| Symbol: |
Tg(Gtf2ird1)KrnJvvw |
| Name: |
transgene insertion Krn, Jeremy Veenstra-VanderWeele |
| MGI ID: |
MGI:7607006 |
| Transgene: |
Tg(Gtf2ird1)KrnJvvw Location: unknown
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| Alliance: |
Tg(Gtf2ird1)KrnJvvw page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Gtf2ird1)KrnJvvw expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Gtf2ird1 (MGI:1861942) |
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Mutation details: A bacterial artificial chromosome which contained the entirety of the 100 kb Gtf2ird1 gene, and 89 kb of flanking regulatory sequence (60 kb at the 5 end and 28 kb at the 3), but no additional intact genes or their promoters was utilized to generate the transgene. This was then recombined using standard methods to insert an HA tag in-frame directly before the stop codon of the beta isoforms 208,209. Homologous recombination via transient expression of RecA was employed, followed by Neomycin selection of an inserted FRT-flanked cassette. The selection cassette was then removed after the expression of FLPe, leaving a single FRT site downstream of the stop codon.
(J:94077)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015; |
| All: |
1 reference(s) |
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Analysis Tools
